| 酵母双杂交系统筛选与水稻黑条矮缩病毒P6互作的水稻蛋白 |
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| 引用本文： | 肖冬来,邓慧颖,谢荔岩,吴祖建,谢联辉.酵母双杂交系统筛选与水稻黑条矮缩病毒P6互作的水稻蛋白[J].热带作物学报,2010,31(3):435-439. |
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| 作者姓名： | 肖冬来 邓慧颖 谢荔岩 吴祖建 谢联辉 |
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| 作者单位： | 福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福建福州,350002 |
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| 基金项目： | 农业部农业公益性行业科研专项项目，教育部博士点专项科研基金，福建省科技厅项目 |
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| 摘 要： | RBSDV编码的非结构蛋白P6为该病毒的沉默抑制子。为了研究P6与寄主水稻间的互作关系,首先利用RT-PCR扩增,获得水稻黑条矮缩病毒P6基因,并将其构建到酵母表达载体pGBKT7上,自激活验证表明,P6蛋白无自激活活性。利用酵母双杂交系统顺序转化法从水稻cDNA文库中筛选,获得4个与RBSDVP6互作的寄主因子,分别为水稻内囊体抗坏血酸过氧化物酶、醛糖1差向异构酶、immutans蛋白基因同源蛋白和一个功能未知蛋白。分析了3个已知功能的寄主因子在病毒侵染过程中的可能功能。
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| 关 键 词： | 黑条矮缩病毒 酵母双杂交 P6蛋白 蛋白互作 |
Screening of Rice Proteins Interacting with P6 of Rice Black Streaked Dwarf Virus from Rice cDNA Library by Yeast Two Hybrid System |
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| Xiao Donglai,Deng Huiying,Xie Liyan,Wu Zujian and Xie Lianhui.Screening of Rice Proteins Interacting with P6 of Rice Black Streaked Dwarf Virus from Rice cDNA Library by Yeast Two Hybrid System[J].Chinese Journal of Tropical Crops,2010,31(3):435-439. |
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| Authors: | Xiao Donglai Deng Huiying Xie Liyan Wu Zujian and Xie Lianhui |
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| Institution: | Institute of Plant Virology, Fujian Agriculture and Forestry University Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University Fujian Key Laboratory for Plant Virology;Institute of Plant Virology, Fujian Agriculture and Forestry University Fujian Key Laboratory for Plant Virology |
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| Abstract: | Rice black streaked dwarf virus(RBSDV)is a plant virus assigned to genus Fijivirus. RBSDV encoded non-structure protein P6 is a viral gene silencing suppressor. In order to find rice proteins interacting with RBSDV P6, P6 was amplified by RT-PCR and cloned into yeast expression vector pGBKT7. Self-activation assay indicated that P6 could not activate itself. Yeast two-hybrid system was used to screen rice cDNA library by sequential transformation method. Four rice proteins were found to interact with P6: three of them shared high homology with thylakoid-bound ascorbate peroxidase, aldose 1-epimerase and immutans protein respectively, and the fourth protein was unknown. Possible functions of the three known rice proteins during RBSDV infection were discussed. |
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| Keywords: | rice black streaked dwarf virus yeast two-hybrid P6 protein protein interaction |
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