| 斗鸡新城疫病毒的分离与生物学特性鉴定 |
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| 引用本文： | 瞿继红,温贵兰,李永明,周碧君,文明,汪德生,龚新勇.斗鸡新城疫病毒的分离与生物学特性鉴定[J].贵州畜牧兽医,2009,33(1):7-9. |
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| 作者姓名： | 瞿继红 温贵兰 李永明 周碧君 文明 汪德生 龚新勇 |
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| 作者单位： | 贵州大学动物科学学院,贵州,贵阳,550025 |
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| 基金项目： | 贵州省自然科学基金，贵州大学人才科研基金 |
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| 摘 要： | 从病死的越南斗鸡脑组织中分离到l株病毒，能凝集鸡的红细胞，而且这种凝集能被新城疫阳性血清特异性抑制。通过对分离株进行RT—PCR检测、动物致病性试验、MDT与ICPI值测定，证实为中等偏强毒力新城疫病毒。动物感染试验表明，20日龄非免疫鸡感染分离毒后24～120h可用RT—PCR方法从喉头和泄殖腔检出新城疫病毒。
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| 关 键 词： | 斗鸡 新城疫病毒 生物学特性 |
Isolation and Biological Characteristics Identification of Newcastle Disease Virus from Game Fowl |
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| Qu Jihong,Wen Guilan,Li Yongming,Zhou Bijun,Wen Ming,Wang Deshen,Gong Xinyong.Isolation and Biological Characteristics Identification of Newcastle Disease Virus from Game Fowl[J].Guizhou Animal Science and Veterinary Medicine,2009,33(1):7-9. |
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| Authors: | Qu Jihong Wen Guilan Li Yongming Zhou Bijun Wen Ming Wang Deshen Gong Xinyong |
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| Institution: | Department of Veterinary Medicine;College of Animal Science;Guizhou University;Guiyang;550025;China |
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| Abstract: | A strain of virus was isolated from the brain of a dead fighting - cock. It was determined to be Newcastle disease virus （NDV） by HI, RT - PCR and negative - contrast electron microscopy. The pathogenicity test values were as follows ： Mean death time（MDT） in healthy embryo was 58.6h, and Intracerebral pathogenicity index （ICPI） in a- day- old chicks was 1.30. Healthy chickens of 3 weeks old was infected after 96h by inocμlating each with 0. 1ml of 1：10 dilution in sterile saline of fresh aUantoic fluid by nose/eyed dropping. The results of animal infection indicated that NDV can be tested after 24h in 20 days old chickens by RT - PCR from the throat and anus. |
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| Keywords: | Fighting-cock Newcastle Disease Biological Characteristics |
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