| 狗牙根品种(系)表型及分子遗传变异研究 |
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| 引用本文： | 谢欢,蒋林峰,马洪平,张新全,刘伟,彭燕.狗牙根品种(系)表型及分子遗传变异研究[J].草业学报,2016,25(3):86-95. |
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| 作者姓名： | 谢欢 蒋林峰 马洪平 张新全 刘伟 彭燕 |
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| 作者单位： | 1.四川农业大学动物科技学院草业科学系,四川 成都611130;2.重庆市垫江县畜牧生产站,重庆408300 |
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| 基金项目： | 四川省“十二五”饲草育种攻关项目(2011NZ0098-11)，现代农业产业技术体系建设专项资金(CARS-35-05)资助。 |
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| 摘 要： | 狗牙根系世界著名多年生禾本科暖季型草坪草,广泛用于不同用途草坪建植.本研究对国内审定的5个狗牙根品种,1个新品系及1个国外引进品种进行表型性状和分子遗传的比较研究,以进一步明确我国狗牙根品种资源的利用特性和遗传基础.结果表明,1)供试狗牙根品种(系)'02011'在试验区域的成坪速度为53 d,绿期为269 d,高于国内审定品种;2)反映株高,匍匐茎,旗叶,花序等特性的9个表型性状在品种间差异均达极显著水平(P<0.01);3)各品种(系)在匍匐茎表面颜色,花序抽出度,叶片上表面颜色,生长习性,生殖枝表面花青甙显色,外颖颖壳,颖尖花青甙显色等植物新品种测试性状上表现出不同程度的群体差异;4)SRAP与SSR分子标记各18对引物分别扩增出167和316条谱带,多态性比例分别为87.51%和70.13%,来自相同地域的供试狗牙根品种具有较近的遗传相似度.
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| 关 键 词： | 狗牙根 表型变异 DUS 性状 遗传多样性 |
| 收稿时间： | 2015-04-29 |
The phenotypic traits and molecular genetic variation of Bermudagrass (Cynodon dactylon)cultivars and breeding lines |
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| XIE Huan,JIANG Lin-Feng,MA Hong-Ping,ZHANG Xin-Quan,LIU Wei,PENG Yan.The phenotypic traits and molecular genetic variation of Bermudagrass (Cynodon dactylon)cultivars and breeding lines[J].Acta Prataculturae Sinica,2016,25(3):86-95. |
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| Authors: | XIE Huan JIANG Lin-Feng MA Hong-Ping ZHANG Xin-Quan LIU Wei PENG Yan |
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| Institution: | 1.Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Chengdu 611130, China;2.Department of Livestock Production Station of Dianjiang, Chongqing 408300, China |
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| Abstract: | Bermudagrass (Cynodon dactylon )is a perennial,warm-season turfgrass well known and widely used for different purposes throughout the world.In order to further clarify the characteristics and genetic basis of C.dactylon germplasm in China,this study performed a comparative study of the phenotypic traits and molec-ular genetics of 5 cultivars and 1 breeding line,as well as 1 cultivar introduced in China.The green stage and turf speed are 53 and 269 d for the breeding line ‘02011’,higher than the 5 cultivars.The differences between cultivars in terms of plant height,flag leaf,creeping stem,and inflorescence characteristics were highly signifi-cant (P <0.01).8 DUS (distinctness,uniformity and stability)characteristics (including stolon surface color, degrees of heading,color of surface blade,reproductive shoot cyanine glucoside color,growth habit,glume cy-anine glucoside color,glume tip cyanine glucoside color)differed among the tested cultivars to varying degrees. 167 and 316 bands were amplified for 18 pairs of primers by SRAP and SSR,of which 87.51% and 70.13%were polymorphic.SRAP and SSR showed that C.dactylon cultivars from the same geographical area could exhibit similar phenotypic traits and relatively close genetic similarities. |
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| Keywords: | Cynodon dactylon phenotypic variation distinctness uniformity and stability (DUS)characters genetic diversity |
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