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| 小麦醇溶 谷蛋白盒结合因子基因表达特性的研究 | |
| 引用本文： | 牛静勇,陈占宽,贺浩华.小麦醇溶 谷蛋白盒结合因子基因表达特性的研究[J].麦类作物学报,2009,29(6):941-946. |
| 作者姓名： | 牛静勇 陈占宽 贺浩华 |
| 作者单位： | 1. 江西农业大学农学院,江西南昌,330045;河南省农作物新品种重点实验室,河南郑州,450002 2. 河南省农作物新品种重点实验室,河南郑州,450002 3. 江西农业大学农学院,江西南昌,330045 |
| 基金项目： | 国家科技支撑计划项目，国家"十一五"科技支撑计划项目，河南省教育厅自然科学研究计划项目 |
| 摘 要： | 为了给小麦品质改良的基因工程研究提供参考依据,以小麦醇溶-谷蛋白盒结合因子(Wheat Prolamin-Box Binding Factor, WPBF)基因为研究对象,从定性和定量两个方面,利用RT-PCR与Real Time PCR系统地研究了WPBF基因在小麦不同组织器官中以及在小麦胚乳整个发育过程中表达的时空特异性.结果表明,WPBF基因的表达是胚乳特异性的,表达跨胚乳的整个发育过程,在其表达的高峰阶段,表达强度为参照基因β-Actin的43%. |
| 关 键 词： | 小麦 醇溶-谷蛋白盒结合因子 |
Study on the Expression Characteristics of Wheat Prolamin Box Binding Factor |
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| NIU Jing yong,CHEN Zhan kuan,HE Hao hua.Study on the Expression Characteristics of Wheat Prolamin Box Binding Factor[J].Journal of Triticeae Crops,2009,29(6):941-946. | |
| Authors: | NIU Jing yong CHEN Zhan kuan HE Hao hua |
| Abstract: | To investigate the expression characteristics of wheat prolamin box binding factor (WPBF) in qualitative and quantitative aspects, we studied the temporal and spatial expression characteristics of WPBF gene in different tissues of wheat during the whole development stage of endosperm using RT-PCR and Real Time PCR methods. The results showed that the expression of WPBF was endosperm-specific; Its expression wasn't detected in roots, stems and leaves at seedling stage, and in roots, stems, leaves and young ears at booting stage. It expressed in the developing endosperm during the whole development stage of endosperm; its expression was weak at 0-4 days after pollination and increased rapidly subsequently, and reached the maximum level of expression at 20 days after pollination, which was 43% of the expression level of the reference gene that was used as β-Actin gene. |
| Keywords: | RT-PCR Real Time PCR Triticum aestivum L WPBF RT-PCR Real Time PCR |
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