| 百喜草耐盐性愈伤组织的生理生化特性研究 |
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| 引用本文： | 孔凡岩,魏凤珍.百喜草耐盐性愈伤组织的生理生化特性研究[J].安徽农业科学,2009,37(33):16320-16321. |
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| 作者姓名： | 孔凡岩 魏凤珍 |
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| 作者单位： | 安徽农业大学农学院,安徽合肥,230036;安徽农业大学农学院,安徽合肥,230036 |
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| 基金项目： | 国家粮科技支撑计划工程项目 |
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| 摘 要： | ［目的］从细胞水平分析比较百喜草耐盐性愈伤组织的生理生化特性。［方法］应用不同浓度（0、5、10、15、20 g/L）的NaCl 处理百喜草成熟种子，诱导产生耐盐的颗粒状愈伤组织和同种普通愈伤组织，研究其含水量、细胞膜透性、MDA含量、POD酶活性、SOD酶活性的变化。［结果］结果表明：随NaCl胁迫浓度的升高,百喜草耐盐组愈伤组织与对照组愈伤组织在多方面存在明显差异,前者含水量、丙二醛含量均高于后者; POD酶活性、SOD酶活性亦均高于对照组。在提高愈伤组织耐盐性诸因素中,脯氨酸起着重要作用。［结论］该研究为进一步筛选百喜草耐盐突变细胞系奠定了良好的基础。
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| 关 键 词： | 百喜草 愈伤组织 耐盐性 脯氨酸含量 |
Physiological and Biochemical Characteristics Study of Salt Tolerance Bahiagrass Callus |
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| KONG Fan-yan et al.Physiological and Biochemical Characteristics Study of Salt Tolerance Bahiagrass Callus[J].Journal of Anhui Agricultural Sciences,2009,37(33):16320-16321. |
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| Authors: | KONG Fan-yan |
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| Institution: | KONG Fan-yan et al(Agronomy College,Anhui Agricultural University,Hefei,Anhui 230036) |
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| Abstract: | Objective]The research aimed to compare the physiological and biochemical characteristics of salt tolerance bahiagrass callus from the cell level.Method]Mature seeds of bahiagrass were dealed with different concentrations(0,5,10,15,20 g/L)of NaCl to induce salt tolerance granular callus and the kinds of general callus.And then the changes of water content,membrane permeability,MDA content,POD activity,SOD activity were studied.Result]The results showed that,with the increase of NaCl concentration,there w... |
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| Keywords: | Bahiagrass Callus Salt tolerance Proline content |
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