| 凝胶色谱净化-气相色谱法测定土壤中六六六和滴滴涕残留 |
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| 引用本文： | 尤继明,李红梅,范明红,杨建涛.凝胶色谱净化-气相色谱法测定土壤中六六六和滴滴涕残留[J].安徽农学通报,2012,18(11):34-36. |
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| 作者姓名： | 尤继明 李红梅 范明红 杨建涛 |
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| 作者单位： | 宝应县产品质量监督检验所,江苏宝应225800／国家有机食品质量监督检验中心,江苏宝应225800;河南农业大学林学院环境系,河南郑州,450002 |
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| 摘 要： | 目的]采用索氏提取和凝胶色谱净化技术,实现了土壤中六六六、滴滴涕8种异构体的气相色谱测定。方法]利用石油醚和丙酮对土壤进行索氏抽提后,旋转蒸发浓缩,进入全自动凝胶色谱净化系统净化后,用气相色谱外标法定量。结果]在此方法下,8种有机氯农药在5～100μg/L范围内呈线性关系,检出限(3S/N)在0.075～0.307μg/kg,加标回收率在90.7%～116.5%。结论]采用凝胶色谱净化-气相色谱法测定土壤中六六六和滴滴涕残留,精密度好,回收率高,检出限较低,能够满足土壤中该农残检测要求。
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| 关 键 词： | 凝胶色谱净化 气相色谱法 六六六 滴滴涕 |
GC Determination of BHC and DDT Residues in Soil after Purification by Gel Permeation Chro- matography |
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| Institution: | You Jiming et al. (1 Baoying Products Quality Supervision And Inspection Institute, Baoying 225800 ;2 National Organic Food Quality Super- vision And Inspection Institute, Baoying 225800) |
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| Abstract: | Objective] GC determination of residual amounts of 8 isomers or homologues of BHC and DDT in soil was a- chieved by separation with Gel permeation chromatography. Method ] Through to the soil Saxhlet extraction with petroleum e- ther and acetone, the extracting solution was concentrated with rotating evaporation, then the liquid was determinated by GC after purified by Gel permeation chromatography. Qualitative analysis was performed based on the retention time, and quanti- tative analysis was carried out by the external standard method. Result ] Linear relationships between values of peak area and concentration of the 8 pesticides were obtained in the same range of 5 to 100 μg/L, with their detection limits （3S/N） in the range of 0. 075 - 0. 307μg/kg. Results of recovery test were found in the range from 90.7% to 116.5%. Conclusion ] This method is good in sensitive and precise, reproducibility and low detection limit, and can be used for the determina- tion of BHC and DDT residues in soil. |
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| Keywords: | Gel permeation chromatography GC determination BHC DDT |
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