| 适于马铃薯茎段再生的植物激素配比选择 |
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| 引用本文： | 栾雨时,徐品三,夏秀英,成之岳.适于马铃薯茎段再生的植物激素配比选择[J].中国马铃薯,2004,18(3):143-144. |
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| 作者姓名： | 栾雨时 徐品三 夏秀英 成之岳 |
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| 作者单位： | 大连理工大学生物科学与工程系,大连,116023 |
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| 摘 要： | 采用正交试验设计,研究了三种植物激素(NAA、BA、GA3)的5个水平对“超白”马铃薯茎段的愈伤组织生长和分化的影响,筛选了一种可一次诱导茎段分化出芽的培养基。试验结果表明,对茎段再生影响最大的植物激素是NAA,其次是BA,再次为GA3。最佳激素配比为NAA0.25mg/L+BA1.5mg/L+GA37mg/L。
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| 关 键 词： | 马铃薯 再生 植物激素 正交试验 |
| 文章编号： | 1672-3635(2004)03-0143-02 |
| 修稿时间： | 2004年2月17日 |
PLANT HORMONE COMBINATIONS SUITABLE FOR POTATO STEM CUTTING REGENERATON |
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| LUAN Yu-shi,XU Pin-san,XIA Xiu-ying,CHENG Zhi-yue.PLANT HORMONE COMBINATIONS SUITABLE FOR POTATO STEM CUTTING REGENERATON[J].Chinese Potato,2004,18(3):143-144. |
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| Authors: | LUAN Yu-shi XU Pin-san XIA Xiu-ying CHENG Zhi-yue |
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| Abstract: | The L25(56)orthogonal design was adopted to find a peculiar plant hormone combination that could make our explants regenerate directly. Three plant hormones(NAA,BA and GA3)were applied at five different levels to study how they influenced the growth and differentiation of “Super White” callus. The results indicated that the most effective plant hormones on stem regeneration was NAA,next was BA,and then GA3. The optimal medium used for stem cutting shoots regeneration had proved to be MS + NAA 0.25 mg/L + BA 1.5mg/L + GA3 7 mg/L. |
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| Keywords: | potato regeneration plant hormones orthogonal design |
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