| 不同类型小豆种质SSR标记遗传多样性及性状关联分析 |
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| 引用本文： | 赵波,叶剑,金文林,曾潮武,吴宝美,濮绍京,潘金豹,万平.不同类型小豆种质SSR标记遗传多样性及性状关联分析[J].中国农业科学,2011,44(4):673-682. |
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| 作者姓名： | 赵波 叶剑 金文林 曾潮武 吴宝美 濮绍京 潘金豹 万平 |
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| 作者单位： | (北京农学院植物科学技术学院农业应用新技术北京市重点实验室); |
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| 基金项目： | 北京市教委人才强教引进人才项目(PXM2007-014207-04453); 北京市教委科研计划面上项目(KM201010020004); 国家自然科学基金项目(31071474) |
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| 摘 要： | 【目的】研究野生、半野生、栽培型小豆的遗传多样性,进一步阐明小豆的起源进化与传播,提高小豆种质的利用效率。【方法】从69对小豆和黑吉豆SSR引物中,筛选出11对多态性好的SSR标记,并结合植株形态性状特征鉴定,对558份来自中国、日本、韩国、不丹、缅甸的野生、半野生和栽培小豆种质资源进行遗传多样性分析和性状关联分析。【结果】共检测到86个等位变异,平均每个位点等位变异数为7.82个,变幅6—10个。野生、半野生、栽培型小豆都有其特征带,栽培小豆的特征带绝大多数来源于中国;野生小豆的特征带仅出现在中国西南、不丹和日本南部地区的种质中。遗传离散度是野生型＞半野生型＞栽培型,半野生型小豆更接近野生型小豆。聚类分析把558个小豆种质分为5大类,归类有较明显的地理相关性和遗传类型的趋同性。日本栽培小豆与韩国和日本野生及半野生小豆亲缘关系近,中国西南野生小豆与东南亚野生材料遗传关系近,与江苏地方品种遗传关系较近。关联分析表明,位于小豆第7连锁群的黑吉豆BG111标记分别解释野生和半野生小豆的主茎节数、茎粗、顶蔓、主茎分枝数性状的49%、44%、31%和18%;栽培小豆中,第1连锁群的黑吉豆BG48、第5连锁群的BG20和第9连锁群的小豆AZ24标记分别解释生育期、株高和单荚粒数、主茎分枝数性状的9%、7%、5%和6%。【结论】野生小豆遗传多样性丰富、变异背景广泛;中国栽培小豆起源于中国,具有丰富的遗传变异。黑吉豆BG111标记与野生和半野生小豆的茎粗、顶蔓、主茎分枝数、主茎节数性状相关联;黑吉豆BG48和BG20、小豆AZ24标记分别与栽培小豆的生育期、株高和单荚粒数、主茎分枝数相关联。
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| 关 键 词： | 小豆 不同类型 SSR 遗传多样性 关联分析 |
| 收稿时间： | 2010-07-02; |
Analysis on Genetic Diversity and Trait Association of Different Types of Azuki Bean(Vigna angularisi)by SSR Markers |
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| ZHAO Bo,YE Jian,JINWen-lin,ZENG Chao-wu,WU Bao-mei,PU Shao jing,PAN Jin-bao,WAN Ping.Analysis on Genetic Diversity and Trait Association of Different Types of Azuki Bean(Vigna angularisi)by SSR Markers[J].Scientia Agricultura Sinica,2011,44(4):673-682. |
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| Authors: | ZHAO Bo YE Jian JINWen-lin ZENG Chao-wu WU Bao-mei PU Shao jing PAN Jin-bao WAN Ping |
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| Institution: | ZHAO Bo,YE Jian,JIN Wen-lin,ZENG Chao-wu,WU Bao-mei,PU Shao-jing,PAN Jin-bao,WAN Ping(Beijing Key Laboratory of New Technology in Agricultural Application,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206) |
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| Abstract: | 【Objective】 It is very important to make studies on the genetic diversity of wild, semi-wild and cultivated azuki beans for further detecting the origin, evolution and diffusion of azuki bean and effectively utilizing these germplasms and serving for azuki bean improvement. 【Method】 Eleven higher polymorphic SSR primers screened from 69 SSR primer pairs of azuki bean and black gram were used in amplification of 558 wild, semi-wild and cultivated azuki bean accessions from China, Japan, South Korea, Bhutan and Burma. The analysis of genetic diversity and trait association was done.【Result】 Eighty-six polymorphic bands were generated. An average of 7.28 polymorphic bands were detected from 1 pair SSR primers. The number of alleles changed from 6 to 10. There were specific bands were observed in wild, semi-wild and cultivated azuki beans. Specific bands of cultivated azuki bean were predominantly produced by Chinese cultivars and landraces. Specific bands of wild azuki bean were detected in accessions from China, Bhutan and South Japan. The order of genetic dispersion was wild＞semi-wild＞cultivated azuki beans. Semi-wild accessions has closer genetic relationship with wild azuki bean. The 558 accessions were divided into five main groups by cluster analysis according to genetic distance. The result of cluster appeared that the genetic types of azuki beans have obviously associated with different geographical regions. Japanese cultivated azuki beans have closer genetic relationship with wild and semi-wild accessions from Japan and South Korea. Wild accessions from Southwest China are closely related to wild accessions from Southeast Asia and landraces of Jiangsu Province. Trait association analysis showed that black gram BG111 marker located on linkage group 7 of azuki bean explained 49%, 44%, 31%, and 18% traits of number of nods on main stem, diameter of stem, top stem twining and branching numbers in wild and weedy types. BG48 of linkage group 1 and BG20 markers of linkage group 5 from black gram and azuki bean AZ24 marker of linkage group 9 accounted for 9%, 7%, 5%, and 6% traits of maturing days, plant height and seed number per pod, number of nods on main stem in cultivated azuki bean respectively.【Conclusion】 A higher genetic variation was detected in wild types than in cultivated accessions. Chinese cultivated azuki bean is originated from China and has the most diversely genetic variation. Number of nods, diameter of stem, top stem twining and branching numbers in wild and semi-wild types are related with black gram BG111 marker. Maturing days, plant height and the number of seeds per pod, number of nods on main stem are associated with black gram BG48 and BG20, azuki bean AZ24 markers in cultivated azuli bean, respectively. |
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| Keywords: | azuki bean(Vigna angularisi) different type SSR genetic diversity trait association analysis |
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