| 温度和时间对血凝试验测定禽流感病毒H5N1亚型Re-6株抗原效价的影响 |
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| 引用本文： | 赵明娟,;赵俊永.温度和时间对血凝试验测定禽流感病毒H5N1亚型Re-6株抗原效价的影响[J].畜牧与饲料科学,2014(7):8-9. |
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| 作者姓名： | 赵明娟 ;赵俊永 |
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| 作者单位： | [1]北京市怀柔区农业局,北京101400; [2]北京市昌平区动物卫生监督管理局城南防疫站,北京102200 |
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| 摘 要： | 旨在研究不同温度和反应时间对血凝试验测定禽流感病毒H5N1亚型Re-6株抗原效价的影响。试验设定4、25、37℃3个温度，分别读取反应5、10、15、20、25、30、35、40、45、50、55、60 min时的抗原效价。结果表明，随着反应温度的升高，试验结果出现时间缩短。4℃反应25 min结果趋于稳定，反应50 min时开始解凝；25℃反应20 min结果趋于稳定，反应40 min时开始解凝；37℃反应15 min结果趋于稳定，反应30 min时开始解凝。
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| 关 键 词： | 温度 时间 血凝试验 抗原效价 |
Effects of Temperature and Time on the Antigen Titer of H5N1 Subtype of Avian Influenza Re-6 Strain by Hemagglutination Test |
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| Institution: | ZHAO Ming-juan, ZHAO Jun-yong (1.Agricuhural Bureau in Huairou District of Beijing, Beijing 101400,China;2.Chengnan Epidemic Prevention Station in Animal Health Supervision and Management Bureau in Changping District of Beijing, Beijing 102200, China) |
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| Abstract: | The research aimed to study the effects of different temperature and reaction time on the antigen titer of H5N1 subtype of avian influenza Re-6 strain determined by hemagglutination test. Three temperatures （4, 25 and 37℃） were set to read the titers after reacting 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 and 60 min respectively. The results showed that the the appearance time of result was shortened with the increase of reaction temperature. The results tended to be stable after reacting at 4℃for 25 min and deflocculation started after reacting 50 min. The results tended to be stable after reacting at 25 ℃ for 20 min and deflocculation started after reacting 40 min. The results tended to be stable after reacting at 37 ℃for 15 min and deflocculation started after reacting 30 min. |
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| Keywords: | temperature time hemagglutination test antigen titer |
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