| 肉鸡肠道NHE2 mRNA表达的组织特异性与发育性变化 |
| |
| 引用本文： | 王修启,邹仕庚,左建军,苏海林,黄志毅,冯定远.肉鸡肠道NHE2 mRNA表达的组织特异性与发育性变化[J].畜牧兽医学报,2008,39(1):42-47. |
| |
| 作者姓名： | 王修启 邹仕庚 左建军 苏海林 黄志毅 冯定远 |
| |
| 作者单位： | 华南农业大学动物科学学院,广州,510642 |
| |
| 基金项目： | 国家重点基础研究发展计划（973）项目（2004CB117501）;国家自然科学基金（30671519） |
| |
| 摘 要： | 选用遗传背景相同的1日龄父母代雄性Arbor Acre（AA）肉雏鸡120羽,随机分为4个重复,采用相对定量RT-PCR方法,以30日龄AA肉鸡肠道RNA为模板,研究肉鸡肠道钠/氢交换载体2（Sodium hydrogen exchanger 2,NHE2）mRNA表达的组织特异性;以AA肉鸡十二指肠和空肠RNA为模板,研究肉鸡肠道NHE2mRNA表达的发育性变化。结果显示：①AA肉鸡十二指肠和空肠NHE2 mRNA的表达丰度显著高于回肠和结直肠（P〈0.05）,而十二指肠和空肠之间、回肠和结直肠之间无显著差异（P〉0.05）;②AA肉鸡NHE2 mRNA在十二指肠及空肠中的表达具有相同的发育模式,2～16日龄升高,30～44日龄下降,55日龄略微回升;在16和30日龄时的表达丰度显著高于2、44和58日龄（P〈0.05）。以上结果说明：AA肉鸡肠道近端NHE2 mRNA的表达丰度显著高于远端（P〈0.05）。AA肉鸡十二指肠及空肠NHE2 mRNA的表达具有相同的发育模式,表明NHE2mRNA表达受到发育阶段的调控,且在十二指肠和空肠间具有稳定性。
|
| 关 键 词： | 肉鸡 肠道 NHE2 mRNA 组织特异性 发育性表达 |
| 文章编号： | 0366-6964（2008）01-0042-06 |
| 收稿时间： | 2007-01-23 |
| 修稿时间： | 2007年1月23日 |
Regional and Ontogenetic Expression of NHE2 mRNA in Intestine of Broiler |
| |
| WANG Xiu-qi,ZOU Shi-geng,ZUO Jian-jun,SU Hai-lin,HUANG Zhi-yi,FENG Ding-yuan.Regional and Ontogenetic Expression of NHE2 mRNA in Intestine of Broiler[J].Acta Veterinaria et Zootechnica Sinica,2008,39(1):42-47. |
| |
| Authors: | WANG Xiu-qi ZOU Shi-geng ZUO Jian-jun SU Hai-lin HUANG Zhi-yi FENG Ding-yuan |
| |
| Abstract: | 120 1-day-old parental male Arbor Acre(AA) broilers were randomly divided into 4 replicates,respectively.Regional expression of sodium hydrogen exchanger 2(NHE2) mRNA in different intestinal tract segments of AA broiler at 30-day-old and ontogenetic expression of NHE2 mRNA in duodenum and jejunum of AA broilers were determined by relative quantitative RT-PCR.It was found that:(1) Expression of NHE2 mRNA in duodenum,jejunum,ileum and colorectum was declined.The abundance of NHE2 mRNA in duodenum and jejunum was higher than that in ileum and colorectum(P<0.05),respectively.(2)Ontogenetic patterns of NHE2 mRNA were same between duodenum and jejunum.The abundance of NHE2 mRNA was increased from 2-to 16-day-old,decreased from 30-to 44-old-day and increased again on 58-day-old.The abundance of NHE2 mRNA on 16-and 30-day-old were higher than that on 2-,44-and 58-day-old(P<0.05),respectively.These results indicate that regional expression of NHE2 mRNA in intestine of AA broilers was significantly declined along the downward intestine from proximal intestine to distal intestine(P<0.05).Ontogeny of NHE2 mRNA in AA broilers had the same pattern in duodenum and jejunum,indicating that NHE2 mRNA expression could be regulated by developmental stage. |
| |
| Keywords: | broiler intestine NHE2 mRNA regional expression ontogenic expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
