| 牛源A型多杀性巴氏杆菌培养特性和免疫原性的研究 |
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| 引用本文： | 姜志刚,马文戈,于力.牛源A型多杀性巴氏杆菌培养特性和免疫原性的研究[J].中国预防兽医学报,2010,32(3). |
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| 作者姓名： | 姜志刚 马文戈 于力 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物病研究室,黑龙江,哈尔滨150001 |
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| 基金项目： | 国家科技支撑计划，黑龙江省科技攻关重大项目 |
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| 摘 要： | 为了确定牛源荚膜血清A型多杀性巴氏杆菌(Pm)的培养特性,本研究采用脑心浸液(BHI)培养基分别于静止、75r/min、250r/min3种培养转速对Pm进行培养,并与加血BHI进行比较,通过绘制生长曲线和小鼠毒力试验对Pm的培养基和培养转速条件进行优化,确定最佳培养条件为,以37℃,0.1%全血脑心浸汤(MB-HI)培养基,75r/min培养16h。将培养的A型Pm灭活后,免疫SPF小鼠,同时设立B型Pm灭活苗免疫组,攻A型Pm后的结果显示,A型灭活菌的保护率为100%,而B型灭活苗免疫组全部死亡。本研究通过对牛源A型Pm培养特性和免疫原性的研究,为新型牛出血性败血症灭活疫苗的研制提供了实验依据。
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| 关 键 词： | 多杀性巴氏杆菌 荚膜血清A型 培养特性 |
Cultural characterization and immunogenicity of bovine capsular serotype A Pasteurella mutocida |
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| JIANG Zhi-gang,MA Wen-ge,YU Li.Cultural characterization and immunogenicity of bovine capsular serotype A Pasteurella mutocida[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(3). |
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| Authors: | JIANG Zhi-gang MA Wen-ge YU Li |
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| Institution: | JIANG Zhi-gang,MA Wen-ge,YU Li (Division of Livestock Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | The Pasteurella mutocida capsular serotype A isolated from affected cattle was passaged under optimized cultured conditions of 37℃,75 r/min,16 h in MBHI medium and monitored by microscopic examination,multiple PCR amplification,CFU determination and virulence test in mice.No significant changes in bacterial morphology,types-specificity,bacterial density and virulence Were observed or detected.The SPF mice were inoculated with inactivated P. Mutocida type A.and P mutocida type B inactivated vaccine was used as a control.The protective rate of the animals by P.mutocida type A was 100%.whereas none of the animals inoculated with P.mutocida type B.were protected.Pathogenicity test showed that P.mutocicla type A had no viru-lence to chicken.Therefore the P.mutocida type A could be developed as a potentially novel Haemorrhagic Septicaemia inactivat-ed vaccine Against P.mutocida. |
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| Keywords: | Pasteurella mutocida capsular serotype A biological characterization |
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