| 吉林省玉米穗腐病致病镰孢菌的鉴定与部分菌株对杀菌剂的敏感性 |
| |
| 引用本文： | 柴海燕,贾娇,白雪,孟玲敏,张伟,金嵘,吴宏斌,苏前富.吉林省玉米穗腐病致病镰孢菌的鉴定与部分菌株对杀菌剂的敏感性[J].中国农业科学,2023,56(1):64-78. |
| |
| 作者姓名： | 柴海燕 贾娇 白雪 孟玲敏 张伟 金嵘 吴宏斌 苏前富 |
| |
| 作者单位： | 1.吉林农业大学植物保护学院，长春 1300222.吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室，长春 130033 |
| |
| 基金项目： | 吉林省重点研发计划(20210202121NC);国家现代农业产业技术体系(CARS-02) |
| |
| 摘 要： | 【目的】明确吉林省玉米穗腐病主要致病镰孢种群分布及杀菌剂对镰孢菌菌丝生长的抑制效果,为针对性地开展玉米镰孢穗腐病的防治提供依据。【方法】通过组织分离法和分子生物学方法对2020年采自吉林省36个市(县)的149份玉米穗腐病样品进行病原菌分离鉴定，利用禾谷镰孢复合种(Fusarium graminearum species complex,FGSC)毒素合成相关基因特异性引物检测其产生毒素的化学型，对部分禾谷镰孢复合种进行致病力测定；采用菌丝生长速率法测定7种杀菌剂对禾谷镰孢复合种的抑制效果。【结果】分离获得233株镰孢菌，隶属4个镰孢复合种，含9种镰孢菌，包括拟轮枝镰孢(F.verticillioides)、布氏镰孢(F.boothii)、禾谷镰孢(F.graminearum)、层出镰孢(F.proliferatum)、亚洲镰孢(F.asiaticum)、厚垣镰孢(F.chlamydosporum)、藤仓镰孢(F.fujikuroi)、木贼镰孢(F.equiseti)和亚黏团镰孢(F.subglutinans)，分离频率依次为33.05%、26.18%、25.32%、12.45%、0....
|
| 关 键 词： | 玉米 穗腐病 镰孢菌 禾谷镰孢复合种 杀菌剂 |
| 收稿时间： | 2022-07-21 |
Identification of Pathogenic Fusarium spp. Causing Maize Ear Rot and Susceptibility of Some Strains to Fungicides in Jilin Province |
| |
| CHAI HaiYan,JIA Jiao,BAI Xue,MENG LingMin,ZHANG Wei,JIN Rong,WU HongBin,SU QianFu.Identification of Pathogenic Fusarium spp. Causing Maize Ear Rot and Susceptibility of Some Strains to Fungicides in Jilin Province[J].Scientia Agricultura Sinica,2023,56(1):64-78. |
| |
| Authors: | CHAI HaiYan JIA Jiao BAI Xue MENG LingMin ZHANG Wei JIN Rong WU HongBin SU QianFu |
| |
| Institution: | 1. College of Plant Protection, Jilin Agricultural University, Changchun 1300222. Institute of Plant Protection, Jilin Academy of Agricultural Sciences/Key Laboratory of Integrated Crop Pest Management in Northeast China, Ministry of Agriculture and Rural Affairs, Changchun 130033 |
| |
| Abstract: | 【Objective】The objective of this study is to clarify the population distribution of Fusarium spp. of maize ear rot in Jilin Province and the inhibitory effect of fungicides on the growth of Fusarium mycelium, and to provide a basis for the targeted control of maize ear rot. 【Method】149 samples of maize ear rot collected from 36 cities and counties in Jilin Province in 2020 were isolated and identified by tissue isolation and molecular biology methods. The specific toxin synthesis primers of related genes were synthesized using Fusarium graminearum species complex (FGSC) toxin. The toxigenic chemotypes were detected, and the pathogenicity of some FGSC was determined. The inhibitory effect of 7 fungicides on FGSC was determined by the mycelial growth rate method. 【Result】A total of 233 Fusarium strains were isolated, belonging to 4 Fusarium complex species, including 9 Fusarium species, which were F. verticillioides, F. boothii, F. graminearum, F. proliferatum, F. asiaticum, F. chlamydosporum, F. fujikuroi, F. equiseti and F. subglutinans. The isolation frequencies were 33.05%, 26.18%, 25.32%, 12.45%, 0.86%, 0.86%, 0.43%, 0.43% and 0.43%, respectively. The isolate frequency of FGSC was the highest, which was 52.36%, and it was the dominant pathogen of maize ear rot in Jilin Province. The proportions of F. boothii, F. graminearum and F. asiaticum in FGSC were 50.00%, 48.36% and 1.46%, respectively. The phylogenetic tree showed that the interspecific and intraspecific genetic diversity of FGSC was rich. The results of pathogenicity assay showed that 52.73% of FGSC were medium pathogenic strains. F. graminearum isolated from the main maize producing areas in the east had the strongest pathogenicity. Toxigenic chemotype detection showed that F. asiaticum produced nivalenol (NIV) chemotype, F. graminearum and F. boothii produced 15-acetyl-deoxynivalenol (15-AcDON) chemotype. The EC50 of the 7 fungicides for inhibiting the growth of FGSC ranged from 0.02 to 19.45 μg·mL-1. Fludioxonil (FS), imazalil (FS), flusilazole (EC), tebuconazole (TC) and myclobutanil (EW) had good inhibitory effects on FGSC and the difference was not significant. The EC50 of FGSC was less than 1.20 μg·mL-1 and EC90 was less than 100 μg·mL-1. The difference of EC50 between F. graminearum and F. boothii was significant under 30% pyraclostrobin treatment. The EC50 of F. graminearum was 10.24 times higher than that of F. boothii. 【Conclusion】The dominant pathogenic Fusarium of maize ear rot in different maize producing areas of Jilin Province is different. F. graminearum and F. boothii are dominant species in the east and west, and F. verticillioides is dominant species in the middle. The interspecific and intraspecific genetic diversity of FGSC is rich. Fludioxonil, imazalil, flusilazole, tebuconazole and myclobutanil have better antifungal effect on FGSC. There is no significant difference in the fungicides susceptibility among FGSC. |
| |
| Keywords: | maize ear rot Fusarium spp Fusarium graminearum species complex (FGSC) fungicide |
|
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
|
