| 不同来源葡萄种质资源果实叶酸含量及ISSR比较分析 |
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| 引用本文： | 梁雎,刘国宏,王保民,何桥,阿布来克·尼亚孜,郭红梅,李兴婷,任红松.不同来源葡萄种质资源果实叶酸含量及ISSR比较分析[J].新疆农业科学,2023,60(1):116-126. |
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| 作者姓名： | 梁雎 刘国宏 王保民 何桥 阿布来克·尼亚孜 郭红梅 李兴婷 任红松 |
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| 作者单位： | 1.新疆农业科学院吐鲁番农业科学研究所,新疆吐鲁番 8380002.中国农业大学,北京 1000833.西南大学,重庆 4007154.新疆农业科学院综合试验场,乌鲁木齐,830000 |
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| 基金项目： | 2021年天山创新团队计划“新疆葡萄种质资源创新利用及栽培技术研究创新团队”(2020D14033);2021年天山青年计划-优秀青年科技人才培养项目“高含量叶酸葡萄品种资源评价和利用”(2020Q023);自治区公益性科研院所基本科研业务经费“葡萄叶酸合成关键基因克隆与分析”(KY2020131) |
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| 摘 要： | 【目的】比较不同来源葡萄果实中叶酸积累的差异，并利用分子标记技术对高叶酸种质资源进行评价和筛选。【方法】采用ELISA技术检测22份葡萄种质资源果实叶酸含量；利用ISSR分子标记对22份葡萄种质资源进行基因组多态性分析和聚类分析；分析检测出叶酸含量与聚类结果。【结果】22份葡萄种质资源果实叶酸含量的范围在1.93～46.95 ng/g,在遗传相似系数为0.621处，22份葡萄材料明显分为2大类群。第1大类叶酸含量平均为35.79 ng/g;其余为第2大类，叶酸含量平均为15.71 ng/g。在遗传相似系数为0.622处，21份葡萄材料又明显分为2大类群。第1大类叶酸含量平均为16.53 ng/g;第2大类叶酸含量平均为12.24 ng/g。欧亚种的叶酸含量较欧美杂种稍高3.998 7 ng/g;从30条引物中筛选出9条扩增稳定且多态性丰富的引物用于葡萄的ISSR扩增。共扩增出78条条带，其中多态性条带70条，多态性百分率为89.7%。22份葡萄种质资源的遗传相似系数为0.474 4～0.846 2,平均遗传相似系数为0.654 5。在遗传相似系数为0.621处，22份葡萄材料明显分为2...
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| 关 键 词： | 不同来源 葡萄种质资源果实 叶酸含量 检测 ISSR |
| 收稿时间： | 2022-05-19 |
Comparative Analysis of Fruit Folic Acid Content and ISSR in Different Grape Germplasm Resources |
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| LIANG Ju,LIU Guohong,WANG Baomin,HE Qiao,Abrake Niazi,GUO Hongmei,LI Xingting,REN Hongsong.Comparative Analysis of Fruit Folic Acid Content and ISSR in Different Grape Germplasm Resources[J].Xinjiang Agricultural Sciences,2023,60(1):116-126. |
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| Authors: | LIANG Ju LIU Guohong WANG Baomin HE Qiao Abrake Niazi GUO Hongmei LI Xingting REN Hongsong |
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| Institution: | 1. Turpan Institute of Agricultural Sciences, Xinjiang Academy of Agricultural Sciences, Turpan Xinjiang 838000, China2. China Agricultural University, Beijing 100083, China3. Southwest University, Chongqing 400715, China4. Comprehensive Test Field of Xinjiang Academy of Agricultural Sciences,Urumqi 830000, China |
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| Abstract: | 【Objective】 To compare the differences of folic acid accumulation in grape fruits from different sources and to evaluate and screen high folic acid germplasm resources by molecular marker technology. 【Methods】 The folic acid content of 22 grape germplasm resources was detected by ELISA; The genomic polymorphism and cluster analysis of 22 grape germplasm resources were carried out by ISSR molecular markers; The detected folic acid content was analyzed with the clustering results. 【Results】 The fruit folic acid content of 22 grape germplasm resources ranged from 1.93 to 46.95 ng/g.Compared with the clustering results, the 22 grape germplasm resources were obviously divided into two groups at the genetic similarity coefficient of 0.621.The average folic acid content of the first category was 35.79 ng/g; The rest belonged to the second category, with an average folate content of 15.71 ng/g.At the genetic similarity coefficient of 0.622, the 21 grape materials were obviously divided into 2 groups.The average folic acid content of the first category was 16.53 ng/g and the average folic acid content of the second category was 12.24 ng/g.At the same time, the analysis of variance showed that the folic acid content of Eurasian species was slightly higher than that of European and American hybrids by 3.998,7 ng/g; Through ISSR molecular marker technology, 9 primers with stable amplification and abundant polymorphism were selected from 30 primers for ISSR amplification of grape.A total of 78 bands were amplified, including 70 polymorphic bands, with a polymorphic percentage of 89.7%.According to the ISSR amplification results, the Jaccard similarity coefficient was analyzed by NTSYSpc2.10e software, and the cluster analysis was carried out by unweighted paired arithmetic mean (UPGMA) to establish the genetic relationship map.The genetic similarity coefficient of 22 grape germplasm resources was between 0.474,4-0.846,2, and the average genetic similarity coefficient was 0.654,5.At the genetic similarity coefficient of 0.621, 22 grape materials were obviously divided into 2 groups.The first category included one Eurasian species (red balado), and the rest was the second category.At the genetic similarity coefficient of 0.622, the 21 grape materials were obviously divided into 2 groups.The first category included 11 Eurasian species (Yanxing seedless, Pinot Grigio, Ruidu Cuixia, Jingmi, Heiqi Seedless, Seedless White, 1124, Jingcui, Ruby, Seedless Purple, Damina), and 6 European and American hybrids (ZA 1, Xiahei, Mihong, Hongbiao Seedless, Luopu Zaosheng and Yuxuan 1).The second category included 4 Eurasian species (Beauty finger, Pink Yadumi, Munag and Rizamat). 【Conclusion】 Through comparing and analyzing the fruit folic acid content and clustering results of different grape germplasm resources, it can be seen that the folic acid content of population 1 is higher than that of population 2.The folic acid content of Eurasian species was slightly higher than that of European and American hybrids. |
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| Keywords: | different sources grape germplasm resources fruit folic acid content detection ISSR |
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