| 燕麦种胚细胞和线粒体AsA-GSH循环对外源H2O2引发的响应 |
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| 引用本文： | 李红玉,王雅聪,夏方山,王勃,李尹琳,张杰敏.燕麦种胚细胞和线粒体AsA-GSH循环对外源H2O2引发的响应[J].草地学报,2023,31(4):1064-1070. |
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| 作者姓名： | 李红玉 王雅聪 夏方山 王勃 李尹琳 张杰敏 |
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| 作者单位： | 山西农业大学草业学院, 山西 太谷 030801 |
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| 基金项目： | 山西农业大学“十四五”生物育种工程项目(YZGC134)；国家自然基金项目(31702171)；山西省重点研发计划项目(201903D221091，202102140601006)；内蒙古大学“省部共建草原家畜生殖调控与繁育”国家重点实验室开放课题(2021KF0303)资助 |
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| 摘 要： | 本研究探讨了外源H2O2引发后，燕麦(Avena sativa)种胚细胞和线粒体抗坏血酸(Ascorbic acid, AsA)-谷胱甘肽(Glutathione, GSH)循环的抗氧化性能的变化规律，以期为植物种子的长期贮藏技术研究提供理论依据。试验以燕麦种子为材料，用浓度为0,0.96,1.92,3.84和7.68 mol·L-1的H2O2引发12 h后，分析其种胚细胞和线粒体脂质过氧化水平、AsA-GSH循环中酶促和非酶促抗氧化物质的变化规律。结果表明：燕麦种胚细胞和线粒体内AsA和GSH含量会随外源H2O2浓度的增加而显著降低(P<0.05),其AsA-GSH循环的酶活性、脱氢抗坏血酸和氧化型谷胱甘肽含量均呈先升后降的趋势，其H2O2和丙二醛含量显著升高(P<0.05),燕麦种子发芽率则显著降低(P<0.05)。燕麦种胚细胞和线粒体AsA-GSH循环的抗氧化功能变化...
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| 关 键 词： | 抗坏血酸-谷胱甘肽循环 线粒体 过氧化氢 燕麦 种子引发 |
| 收稿时间： | 2022-11-08 |
Response of AsA-GSH Cycles to Exogenous H2O2 Priming in the Embryonic Cells and Mitochondria of Oat Seeds |
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| LI Hong-yu,WANG Ya-cong,XIA Fang-shan,WANG Bo,LI Yin-lin,ZHANG Jie-min.Response of AsA-GSH Cycles to Exogenous H2O2 Priming in the Embryonic Cells and Mitochondria of Oat Seeds[J].Acta Agrestia Sinica,2023,31(4):1064-1070. |
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| Authors: | LI Hong-yu WANG Ya-cong XIA Fang-shan WANG Bo LI Yin-lin ZHANG Jie-min |
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| Institution: | College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China |
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| Abstract: | In this study,the changes in the antioxidant properties of ascorbic acid (AsA)-glutathione (GSH) cycles were explored as a response to exogenous H2O2 priming in the embryonic cells and mitochondria of oat (Avena sativa) seeds,which provided a theoretical basis for the long-term storage of plant seeds. Oat seeds were primed with 0,0.96,1.92,3.84 and 7.68 mol·L-1 H2O2 for 12 h as the experimental materials,and the changes in lipid peroxidation and enzymatic and non-enzymatic antioxidants in AsA-GSH cycles were analyzed in both their embryonic cells and mitochondria. The results showed that the AsA and GSH contents were significantly decreased in the embryonic cells and mitochondria (P<0.05),and enzymatic activities of AsA-GSH cycles and contents of dehydroascorbate and oxidized glutathione were all with a pattern of increasing firstly and then decreasing. H2O2 and malondialdehyde contents were also significantly increased (P<0.05),and thus the germination percentage of oat seeds was reduced significantly (P<0.05). Embryonic cells and mitochondria showed similar results of changes of AsA-GSH cycles in response to the increase of H2O2 concentration,and the damage on their antioxidant capacity resulted in the decrease of seed germination under priming with high concentration (≥1.92 mol·L-1) of exogenous H2O2. It might be caused largely by the decrease of AsA content and its continuous production. |
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| Keywords: | Ascorbic acid-glutathione cycles Mitochondria Hydrogen peroxide Oat Seed priming |
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