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摘叶和喷施外源过氧化氢对库尔勒香梨粗皮果形成的影响
引用本文:史智勇,艾沙江·买买提,仙米斯娅·塔依甫,曹艺洁,玉苏甫·阿不力提甫.摘叶和喷施外源过氧化氢对库尔勒香梨粗皮果形成的影响[J].新疆农业科学,2023,60(4):889-896.
作者姓名:史智勇  艾沙江·买买提  仙米斯娅·塔依甫  曹艺洁  玉苏甫·阿不力提甫
作者单位:1.新疆农业大学林学与园艺学院,乌鲁木齐 8300522.新疆农业科学院园艺作物研究所,乌鲁木齐 8300913.新疆维吾尔自治区农药检定所,乌鲁木齐 830049
基金项目:新疆维吾尔自治区自然科学基金项目(2019D01A63);新疆维吾尔自治区园艺重点学科(2016-10758-3);新疆农业大学特色林果果实发育与调控创新团队
摘    要:【目的】研究库尔勒香梨上可能诱导粗皮果形成的胁迫因子。【方法】基于分析库尔勒香梨粗皮果形成的基础上,对生长期的库尔勒香梨枝条采用50%程度地摘叶、外源喷施250 mmol/L的H2O22种人为逆境处理,在成熟期统计处理枝条上粗皮果率,对不同处理的果实进行组织切片染色,检测生理指标。【结果】库尔勒香梨成熟期对照组的粗皮果比率为18.10%,H2O2喷施和摘叶处理的粗皮果率分别为51.93%、29.52%;3组果实中果皮中的木质素含量分别为3.4%、4.9%、3.9%,果肉中的木质素含量分别为1.5%、1.7%、2.0%,2个处理组均提高了库尔勒香梨的粗皮果率以及果实中的木质素含量。H2O2喷施后外果皮的丙二醛(MDA)和活性氧(H2O2和O-2)含量显著增加,分别为对照的1.7和2.9倍。与之相应,库尔勒香梨果皮可溶性糖、淀粉等渗透调节物质含量也显著增加,分别为对照的1....

关 键 词:库尔勒香梨  粗皮果  氧化胁迫  高温复合胁迫
收稿时间:2022-09-10

Study on the induction of rough-skinned fruit formation in Korla fragrant pear
SHI Zhiyong,Aisajan Mamat,Xianmisiya Tayifu,CAO Yijie,Yusuf Ablitif.Study on the induction of rough-skinned fruit formation in Korla fragrant pear[J].Xinjiang Agricultural Sciences,2023,60(4):889-896.
Authors:SHI Zhiyong  Aisajan Mamat  Xianmisiya Tayifu  CAO Yijie  Yusuf Ablitif
Institution:1. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China2. Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China3. Institute of the Control of Agrochemicals of Xinjiang Uygur Autonomous Region, Urumqi 830049, China
Abstract:【Objective】This project aims to explore the stress factors that may induce the formation of rough-skinned fruits on Korla fragrant pear. 【Methods】Based on the previous analysis on the formation of rough-skinned fruits, through two artificial stress treatments (leaf picking and exogenous H2O2 spraying) were performed on the bearing shoots of Korla fragrant pear in the growth period; The rates of rough-skinned fruits, the tissue section staining and the physiological indexes testing were carried out at the maturity stage. 【Results】The ratio of rough-skinned fruit in the control group, H2O2 spraying group and the leaf picking group were 18.10%, 51.93% and 29.52%, respectively. In the three groups, the lignin contents in pericarp were 3.4%, 4.9% and 3.9% respectively, and the lignin contents in pulp were 1.5%, 1.7% and 2.0% respectively. Leaf picking and H2O2 spraying increased the rates of rough-skinned fruits and lignin content. In addition, malondialdehyde (MDA) and reactive oxygen species(H2O2 and $O^{-}_{2}$)increased significantly after H2O2 spraying, which were 1.7 and 2.9 times of those in the control, respectively. Corresponding to it, the contents of osmoregulation substances such as soluble sugar and soluble starch in the pericarp were 1.3-fold and 1.4-fold higher than those in control group. After leaf picking, the content of soluble sugar, starch and other osmotic regulatory substances in the pear pulp were also significantly increased, which were 1.2 and 2.0 times higher than those in the control group, respectively. 【Conclusion】Both leaf picking and H2O2 spraying can effectively induce the formation of rough-skinned fruits by increasing the number of stone cells and lignin content in the fruit. Among them, H2O2 spraying group mainly affects the peel part of Korla fragrant pear while leaf picking mainly affects the pulp part.
Keywords:Korla fragrant pear  rough-skinned fruit  oxidative stress  combined stress of high temperature and light  
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