| 芒果MiZAT10A和MiZAT10B功能分析 |
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| 引用本文： | 莫文静,朱嘉伟,何新华,余海霞,江海玲,覃柳菲,张艺粒,李雨泽,罗聪.芒果MiZAT10A和MiZAT10B功能分析[J].中国农业科学,2023,56(1):193-202. |
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| 作者姓名： | 莫文静 朱嘉伟 何新华 余海霞 江海玲 覃柳菲 张艺粒 李雨泽 罗聪 |
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| 作者单位： | 广西大学农学院/亚热带农业生物资源保护与利用国家重点实验室/植物科学国家级实验教学示范中心，南宁 530004 |
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| 基金项目： | 广西自然科学基金(2015GXNSFAA139052);国家现代农业产业技术体系广西芒果创新团队栽培与病虫害防治岗位项目(nycytxgxcxtd-2021-06-02);科技先锋队‘强农富民’‘富六个一’专项行动(202204) |
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| 摘 要： | 【目的】锌指蛋白（zinc finger protein,ZFP）在植物非生物胁迫应答中起重要的作用，研究两个锌指蛋白基因MiZAT10A和MiZAT10B转入拟南芥对盐、干旱、重金属以及外源激素等非生物胁迫的应答，为抗逆育种提供理论依据。【方法】利用在线软件PLACE和MEME分别对芒果MiZAT10A和MiZAT10B进行启动子顺式作用元件以及motif预测和分析，并利用TBtools软件和‘四季蜜芒’基因注释文件（GFF文件，未公开）绘制染色体定位图；通过实时荧光定量分析MiZAT10A和MiZAT10B的组织表达模式；构建芒果MiZAT10A和MiZAT10B超量表达载体，采用农杆菌花序浸染法转化模式植物拟南芥，观察并记录转基因拟南芥开花表型以及在盐、干旱、重金属以及外源激素脱落酸和赤霉素处理下的根生长情况。【结果】启动子顺式元件分析显示，两个基因的启动子区域都有许多光响应元件、激素响应元件和非生物胁迫响应元件。表达模式分析显示，MiZAT10A与MiZAT10B在芽和花中表达水平最高。MiZAT10A和MiZAT10B分别获得了9株和14株转基因拟南芥，开花表型分析显示，Mi...
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| 关 键 词： | 芒果 非生物胁迫 锌指蛋白 表达模式 基因功能分析 |
| 收稿时间： | 2022-03-02 |
Functional Analysis of MiZAT10A and MiZAT10B Genes in Mango |
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| MO WenJing,ZHU JiaWei,HE XinHua,YU HaiXia,JIANG HaiLing,QIN LiuFei,ZHANG YiLi,LI YuZe,LUO Cong.Functional Analysis of MiZAT10A and MiZAT10B Genes in Mango[J].Scientia Agricultura Sinica,2023,56(1):193-202. |
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| Authors: | MO WenJing ZHU JiaWei HE XinHua YU HaiXia JIANG HaiLing QIN LiuFei ZHANG YiLi LI YuZe LUO Cong |
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| Institution: | College of Agriculture, Guangxi University/State Key Laboratory of Subtropical Agricultural Biological Resources Protection and Utilization/National Experimental Teaching Demonstration Center of Plant Science, Nanning 530004 |
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| Abstract: | 【Objective】Zinc finger protein (ZFP) plays an important role in plant abiotic stress response. Therefore, to provide a theoretical basis for stress resistance breeding, this study aimed to analyze the response of two zinc finger protein genes of MiZAT10A and MiZAT10B transgenic Arabidopsis to abiotic stresses, such as salt, drought, heavy metals and exogenous hormones. 【Method】 The promoter cis acting elements and motif of mango MiZAT10A and MiZAT10B genes were predicted and analyzed by online software PLACE and MEME, respectively. The chromosome location map was drawn by TBtools software and SiJiMi gene annotation file (GFF file and unpublished). Tissue expression patterns of MiZAT10A and MiZAT10B genes were analyzed by qRT-PCR. The overexpression vectors of MiZAT10A and MiZAT10B genes were constructed and transformed into Arabidopsis thaliana by Agrobacterium floral-dip method. The phenotype of MiZAT10A and MiZAT10B transgenic plant were observed and recorded under salt, drought, heavy metals, abscisic acid and gibberellin treatments. 【Result】 Promoter cis element analysis showed that there were many light response elements, hormone response elements and abiotic stress response elements in the promoter region of MiZAT10A and MiZAT10B genes. Expression analysis showed that MiZAT10A and MiZAT10B were highly expressed in buds and flowers. 9 of MiZAT10A and 14 of MiZAT10B transgenic Arabidopsis strains were obtained. Overexpression of MiZAT10A and MiZAT10B significantly resulted early flowering compared with the control lines. The root length of MiZAT10A and MiZAT10B overexpressing transgenic Arabidopsis was significantly longer than that of control lines under salt stress, drought stress, heavy metal stress, GA3 and ABA hormone treatments. 【Conclusion】 Overexpression of MiZAT10A and MiZAT10B not only promoted transgenic Arabidopsis flowering early but also improved salt, drought, heavy metals and exogenous hormones GA3 and ABA resistance. |
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| Keywords: | mango abiotic stress zinc finger protein expression function analysis |
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