| 基于LC/MS的伊犁马3600 m速度赛赛前、赛后血浆代谢组学差异变化 |
| |
| 引用本文： | 张仕琦,李晓斌,张文杰,韩明,王世昌,郑文祥,欧阳文,祁居中,杨开伦.基于LC/MS的伊犁马3600 m速度赛赛前、赛后血浆代谢组学差异变化[J].新疆农业科学,2023,60(2):501-510. |
| |
| 作者姓名： | 张仕琦 李晓斌 张文杰 韩明 王世昌 郑文祥 欧阳文 祁居中 杨开伦 |
| |
| 作者单位： | 1.新疆农业大学动物科学学院/新疆肉乳用草食动物营养重点实验室,乌鲁木齐 8300522.新疆伊犁哈萨克自治州昭苏县种马场,新疆昭苏 835000 |
| |
| 基金项目： | 新疆维吾尔自治区“十三五”重大专项-运动马健康养殖共性技术研发(2017A01002-2) |
| |
| 摘 要： | 【目的】研究伊犁马3 600 m速度赛赛前赛后血浆中代谢物的差异变化。【方法】根据马场提供的遗传系谱，挑选5匹(3♂+2♀)无亲缘关系、体重(384.40±34.18)kg、年龄为2岁的速度赛用伊犁马，每日进行等强度训练。第25 d晨饲后4 h进行3 600 m速度赛，试验马以(11.75±0.87)m/s的平均速度完成比赛。赛前安稳状态下和赛后即刻测定心率和呼吸频率，采集血液、制备血浆样品，采集血浆的质谱，采用非靶向性代谢组学方法测定代谢物。【结果】与赛前心率(39.80±0.40)bpm、呼吸频率(14.00±1.80)bpm相比，3 600 m速度赛赛后即刻试验马心率(80.80±7.70)bpm、呼吸频率(78.80±3.90)bpm显著升高(P<0.05)。与赛前相比，试验马赛后即刻部分血浆代谢物产生显著变化，差异代谢物主要涉及柠檬酸循环、丙氨酸、天冬氨酸和谷氨酸代谢、五糖和葡萄糖醛酸间转化、半胱氨酸与蛋氨酸代谢、氧化磷酸化、脂肪酸降解、鞘脂代谢等生化代谢途径(P<0.05)。【结论】伊犁马3 600 m速度赛前、赛后血浆样本的LC/MS代谢谱存在差异，差异代谢物...
|
| 关 键 词： | 伊犁马 3 600 m速度赛 运动前后 非靶向代谢组学 血浆代谢差异物 |
| 收稿时间： | 2022-02-20 |
LC/MS-Based Metabolomics Approach for Exploring Plasma Metabolome Modififications before and after 3,600 m Speed Race in Yili horse |
| |
| ZHANG Shiqi,LI Xiaobin,ZHANG Wenjie,HAN Ming,WANG Shichang,ZHENG Wenxiang,OUYANG Wen,QI Juzhong,YANG Kailun.LC/MS-Based Metabolomics Approach for Exploring Plasma Metabolome Modififications before and after 3,600 m Speed Race in Yili horse[J].Xinjiang Agricultural Sciences,2023,60(2):501-510. |
| |
| Authors: | ZHANG Shiqi LI Xiaobin ZHANG Wenjie HAN Ming WANG Shichang ZHENG Wenxiang OUYANG Wen QI Juzhong YANG Kailun |
| |
| Institution: | 1. Xinjiang Key Laboratory of Meat&Milk Production Herbivore Nutrition/College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China2. Zhaosu County, Yili Kazakh Autonomous Prefecture, Zhaosu Xinjiang 835000, China |
| |
| Abstract: | 【Objective】 The aim of this study is to investigate the difference and change of plasma metabolites before and after 3,600 m speed race in Yili horses. 【Method】 According to the genetic pedigree provided by the equestrian club, 5(3♂2♀) unrelated, weight (384.40±34.18)kg, and age 2 speed raced-horses were selected.The horses were trained daily with equal intensity. On the 25 th day of the experimental stage, the 3,600 m speed race was performed 4 h after morning feeding. The horse completed the race at (11.75±0.87) m/s at average speed. The rate of heartbeat and the breathing were measured immediately before and after the race. At the same time, blood was collected, plasma samples were prepared, plasma mass spectrometry was collected, and metabolites were determined by non-target metabolomics method. 【Results】 Compared with the pre-competition heart rate (39.80±0.40) bpm and respiratory rate (14.00±1.80) bpm, the horse heart rate (80.80±7.70) bpm and respiratory rate (78.80±3.90) bpm were significantly increased (P<0.05). Compared with Yili horses plasma at pre-race, the citric acid cycle, metabolism of alanine, aspartic acid and glutamic acid, transformation between pentaccharide and glucuronic acid, metabolism of cystine and methionine, oxidative phosphorylation, fatty acid degradation and sheath lipid metabolism showed significant changes. On the basis of the LC/MS analysis of plasma metabolomics of Yili horse before and after the 3,600 m speed race, LC/MS metabolic profiles of plasma samples of Yili horse 3,600 m before and after the race were different. The differential metabolites involved in citric acid cycle, glycolysis system, fat metabolism, amino acid metabolism. 【Conclusion】 During the 3,600 m speed race, the Yili horse body presents anaerobic, aerobic and mixed oxygen metabolism characteristics show anaerobic, aerobic and mixed oxygen metabolism. |
| |
| Keywords: | Yili horse 3 600 m speed race before and after speed race non-targeted metabolomics plasma metabolites |
|
| 点击此处可从《新疆农业科学》浏览原始摘要信息 |
| 点击此处可从《新疆农业科学》下载免费的PDF全文 |
|
