| 桃色欧文氏菌Cp2脂多糖对紫花苜蓿幼苗生长的影响 |
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| 引用本文： | 张宏,黄荣,姚博,张玉娟,张振粉.桃色欧文氏菌Cp2脂多糖对紫花苜蓿幼苗生长的影响[J].草地学报,2023,31(2):615-622. |
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| 作者姓名： | 张宏 黄荣 姚博 张玉娟 张振粉 |
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| 作者单位： | 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃 兰州 730070 |
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| 基金项目： | 国家自然科学基金(32060369)；兰州市人才创新创业项目(2019-RC-116)；甘肃农业大学青年导师基金项目(GAU-QDFC-2019-08)；甘肃省杰出青年基金(20JR10RA562)；草业生态系统教育部重点实验室-人才培育项目(KLGE202203)资助 |
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| 摘 要： | 为探究桃色欧文氏菌脂多糖(Erwinia persicina Cp2 lipopolysaccharides, Cp2-LPSe)对紫花苜蓿(Medicago sativa)幼苗生长的影响，本研究以紫花苜蓿‘巨能551’为材料，用改良的热酚水法提取纯化Cp2-LPSe,设不同浓度(0,0.157,0.314,0.471和0.628 EU·mL-1)Cp2-LPSe处理，测定处理后种子发芽和幼苗生长指标。结果表明：改良后的热酚水法提取Cp2-LPSe的平均产率为4.27%;不同Cp2-LPSe浓度下，紫花苜蓿种子的发芽率、发芽势和发芽指数均低于对照，且发芽指数显著低于对照(P<0.05);随着Cp2-LPSe浓度的升高，幼苗的各生长指标均呈先升后降趋势，当Cp2-LPSe浓度为0.157 EU·mL-1时，各指标均达到最大值，且显著高于对照(P<0.05)。相关性和主成分分析结果表明，0.157 EU·mL-1Cp2-LPSe对紫花苜蓿幼苗生长有较大影响。综上，0.157 EU·mL-1 ...
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| 关 键 词： | 桃色欧文氏菌脂多糖 紫花苜蓿 幼苗生长 相关性分析 |
| 收稿时间： | 2022-08-17 |
Effect of Lipopolysaccharide in Erwinia persicina Cp2 on the growth of Medicago sativa seedlings |
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| ZHANG Hong,HUANG Rong,YAO Bo,ZHANG Yu-juan,ZHANG Zhen-fen.Effect of Lipopolysaccharide in Erwinia persicina Cp2 on the growth of Medicago sativa seedlings[J].Acta Agrestia Sinica,2023,31(2):615-622. |
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| Authors: | ZHANG Hong HUANG Rong YAO Bo ZHANG Yu-juan ZHANG Zhen-fen |
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| Institution: | Pratacultural College, Gansu Agricultural University;Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Center for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China |
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| Abstract: | To investigate the effect of Erwinia persicina Cp2 Lipopolysaccharides (Cp2-LPSe) on the growth of alfalfa seedlings,in this experiment,alfalfa 'Juneng 551' was used to extract and purify Cp2-LPSe based on the improved hot phenol water method. Alfalfa was treated with 0,0.157,0.314,0.471,and 0.628 EU·mL-1 Cp2-LPSe,and seed germination and seedling growth were investigated. The results showed that the average yield of Cp2-LPSe was 4.27%. The germination percentage,germination potential,and germination index of alfalfa seeds treated with different Cp2-LPSe concentrations were lower than those of the control,and the germination index was significantly lowered (P<0.05). With the increase of Cp2-LPSe concentration,all the growth indexes of seedlings increased at first and then decreased. With 0.157 EU·mL-1 Cp2-LPSe treated,all the germination and growth indexes reached the maximum and were significantly higher than those of the control (P<0.05). The correlation and principal component analysis confirmed that 0.157 EU·mL-1 Cp2-LPSe had a significant effect on the growth of alfalfa seedlings. In conclusion,0.157 EU·mL-1 Cp2-LPSe treatment promoted the growth of alfalfa seedlings,and was beneficial to the accumulation of biomass,but more than this concentration would inhibit the growth. This study laid a practical and theoretical foundation for the development of alfalfa growth-promoting reagent Cp2-LPSe. |
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| Keywords: | Erwinia persicina Cp2 lipopolysaccharides Medicago sativa Seedling growth Correlation analysis |
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