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优良绣球品种“尼克兰”培养技术研究
引用本文:韩玉林.优良绣球品种“尼克兰”培养技术研究[J].安徽农业科学,2008,36(29).
作者姓名:韩玉林
作者单位:江西财经大学资源与环境学院,江西南昌,330032
摘    要:目的]提高优良绣球属花卉品种"尼克兰"的繁殖速度。方法]选用"尼克兰"健壮幼嫩茎尖作为组培试验材料,进行组培快繁的研究。结果]结果表明:诱导愈伤组织形成的培养基组合为MS+BA 2.0 mg/L+NAA 0.1 mg/L,有利于愈伤组织生长;不定芽分化的培养基为MS+BA 2.0 mg/L+NAA 0.1 mg/L,有利于不定芽的分化;而4/5MS+BA 2.0 mg/L+NAA 0.1 mg/L有利于组培苗不定根的形成。结论]在"尼克兰"的组培繁殖中,重要的是注意茎尖和环境的消毒。

关 键 词:绣球品种  组织培养  适应性

Study on the Plant Tissue Culture Technology of Hydrangea macrophylla 'Nikko Blue'
HAN Yu-lin.Study on the Plant Tissue Culture Technology of Hydrangea macrophylla 'Nikko Blue'[J].Journal of Anhui Agricultural Sciences,2008,36(29).
Authors:HAN Yu-lin
Abstract:Objective] The study aimed to improve the breeding speed of Hydrangea macrophylla 'Nikko Blue'.Method] Vigorous and healthy shoot tips of H.macrophylla 'Nikko Blue' was selected as explants,and used to carry out the plant tissue culture technology research.Result] The results showed that plant hormone combination of MS+BA 2.0 mg/L + NAA 0.1 mg/L was good for callus induction,MS+BA 2.0 mg/L + NAA 0.1 mg/L was suitable for adventitious bud formation,4/5 MS + BA 2.0 mg/L + NAA 0.1 mg/L was good for root formation.Conclusion] During the tissue culture of H.macrophylla 'Nikko Blue',the disinfection of shoot tip and environment was very important.
Keywords:Hydrangea macrophylla 'Nikko Blue'  Tissue culture  Adaptability
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