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| 奶牛乳房炎抗性相关基因Znf313的电子克隆与序列分析 | |
| 引用本文： | 曹随忠,李宏滨,姚学萍,赵兴绪,杜立新.奶牛乳房炎抗性相关基因Znf313的电子克隆与序列分析[J].中国农学通报,2007,23(1):6-006. |
| 作者姓名： | 曹随忠 李宏滨 姚学萍 赵兴绪 杜立新 |
| 作者单位： | 1. 中国农业科学院畜牧研究所,北京,100094;甘肃农业大学动物医学院,兰州,730070;四川农业大学动物科技学院,雅安,625014 2. 中国农业科学院畜牧研究所,北京,100094 3. 四川农业大学动物科技学院,雅安,625014 4. 甘肃农业大学动物医学院,兰州,730070 |
| 基金项目： | 国家奶业重大科技专项“奶牛产奶性能分子遗传标记的筛选”（No.2002BA518A01.13）;科技部国际科技合作重点项目计划“奶牛乳房炎抗性大的功能基因组学及蛋白质组学研究”（No.2005DFA30720）. |
| 摘 要： | 电子克隆是基因克隆的新策略。利用一个患乳房炎奶牛外周血白细胞中差异表达的EST序列为信息探针进行电子克隆，根据电子克隆序列设计引物，以奶牛外周血白细胞cDNA为模板进行RT-PCR验证，PCR产物克隆入pGEM-T质粒载体，测序得到的序列已被GenBank收录（DQ010409）。该基因被命名为牛锌指蛋白313(Znf313)基因，开放阅读框1~693bp，推测编码230个氨基酸，预测其编码蛋白分子量为25.67kD，等电点6.90。 |
| 关 键 词： | 姜瘟病 姜瘟病 生物防治 拮抗细菌 抑菌圈 |
| 修稿时间： | 2006-06-26 |
In Silico Cloning and Characterization of Mastitis Resistant Gene Znf313 from Dairy Cows |
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| Cao Suizhong,Li Hongbin,Yao Xueping,Zhao Xingxu,Du Lixin.In Silico Cloning and Characterization of Mastitis Resistant Gene Znf313 from Dairy Cows[J].Chinese Agricultural Science Bulletin,2007,23(1):6-006. | |
| Authors: | Cao Suizhong Li Hongbin Yao Xueping Zhao Xingxu Du Lixin |
| Institution: | (1Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094; 2College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070; 3Animal Science & Technology College, Sichuan Agricultural University, Yaan Sichuan 625 |
| Abstract: | In silico cloning was a new strategy of gene cloning developed with the development of genome,EST projects and bio-informatics.Using one differentially expressed EST in peripheral blood leukocytes of Holstein cows with mastitis as a querying probe,a novel cDNA sequence was obtained from bovine EST database of GenBank,which was named bovine zinc finger protein 313(Znf313) gene.The sequence of in silico cloning was verified by RT-PCR,and inserted into pGEM-T vector for sequencing.The bovine Znf313 gene cDNA contains a 693bp open reading frame,encoding 230aa protein,putative protein molecular weight is 25.67kD,isoelectric point is 6.90.The sequence has been submitted to GenBank with the accession number DQ010409. |
| Keywords: | Bovine Znf313 gene In silico cloning Bio-informatics Mastitis resistance |
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