| 7种鸭源细菌的分离与16S rDNA测序鉴定 |
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| 引用本文： | 孙化露,陈冰,李树纯,陈素娟,彭大新.7种鸭源细菌的分离与16S rDNA测序鉴定[J].中国兽医杂志,2011,47(10). |
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| 作者姓名： | 孙化露 陈冰 李树纯 陈素娟 彭大新 |
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| 作者单位： | 扬州大学兽医学院农业部畜禽传染病学重点开放实验室,江苏扬州,225009 |
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| 基金项目： | 公益性行业(农业)专项经费资助，国家自然科学基金，青蓝工程 |
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| 摘 要： | 从病死鸭的脏器中无菌分离细菌.根据培养特性、菌落形态、革兰染色和生化特性,鉴定出7种细菌分离株.以分离株的基因组DNA为模板,用16S rDNA试剂盒扩增其DNA片段,测序后NCBI网站进行BLAST搜索比对,鉴定出细菌种类,分别为鼠伤寒沙门菌、大肠杆菌、鸭疫里默氏杆菌、铜绿假单胞杆菌、施氏假单胞菌、浅绿气球菌和麦氏棒杆菌.前3种为鸭的常见分离菌,其余的较为少见.因此在鸭病控制过程中应注意可能存在一些不常见细菌的混合感染.
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| 关 键 词： | 鸭 细菌 致病 16S rDNA |
Isolation and identification by 16S rDNA analysis of seven bacteria from ducks |
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| SUN Hua-lu,CHEN Bing,LI Shu-chun,CHEN Su-juan,PENG Da-xin.Isolation and identification by 16S rDNA analysis of seven bacteria from ducks[J].Chinese Journal of Veterinary Medicine,2011,47(10). |
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| Authors: | SUN Hua-lu CHEN Bing LI Shu-chun CHEN Su-juan PENG Da-xin |
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| Institution: | SUN Hua-lu,CHEN Bing,LI Shu-chun,CHEN Su-juan,PENG Da-xin(College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | Bacteria were isolated aseptically from organs of diseased ducks.Seven species of bacterial strains were identified by cultural and biochemical characteristics,morphological observation of colonies,and Gram staining.Seven 16s rDNA fragments were PCR amplified from chromosomal DNA of these strains.The results of 16s rDNA sequence analysis by BLAST in GenBank indicated that they were Salmonella typhimurium,Escherichia coli,Riemerella anatipestifer,Pseudomonas aeruginosa,Pseudomonas stutzeri,Aerococcus viridan... |
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| Keywords: | duck bacterium pathopoiesis 16s rDNA |
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