| 长白山笃斯越桔优良单株组培快繁技术的研究 |
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| 引用本文： | 宗长玲,宗成文,赵巍巍,金炳奎,曹后男.长白山笃斯越桔优良单株组培快繁技术的研究[J].北方园艺,2012(4):113-116. |
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| 作者姓名： | 宗长玲 宗成文 赵巍巍 金炳奎 曹后男 |
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| 作者单位： | 延边大学农学院,吉林延吉,133002 |
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| 基金项目： | 国家自然科学基金资助项目(30960231) |
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| 摘 要： | 以长白山笃斯越桔优良单株的茎尖、茎段为外植体,对其组培快繁技术进行了研究。结果表明:笃斯越桔的幼芽分化适宜的启动培养基为WPM+ZT 2.0mg/L;增殖培养基为改良MS+ZT 0.5mg/L+IBA 0.15mg/L,继代培养30d后的增殖倍数可达5.3倍;适宜的生根培养基为1/4改良MS+IBA 0.5mg/L,培养50d后的生根率可达80%。将笃斯越桔组培苗在纯沙子中练苗20d,将苗移栽至草炭土∶腐殖土为1∶2的基质中,组培苗移栽成活率达78%。
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| 关 键 词： | 笃斯越桔 优良单株 组织培养 快速繁殖 |
In vitro Culture of Superior Individual of Vaccinium uliginosum from Changbai Mountain |
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| ZONG Chang-ling
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ZONG Cheng-wen
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ZHAO Wei-wei
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JIN Bing-kui
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CAO Hou-nan.In vitro Culture of Superior Individual of Vaccinium uliginosum from Changbai Mountain[J].Northern Horticulture,2012(4):113-116. |
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| Authors: | ZONG Chang-ling ZONG Cheng-wen ZHAO Wei-wei JIN Bing-kui CAO Hou-nan |
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| Institution: | (College of Agriculture,Yanbian University,Yanji,Jilin 133002) |
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| Abstract: | Taking stem or shoot tip of Vaccinium uliginosum as the explants,in vitro culture of superior individual of Vaccinium uliginosum distributing in Changbai Mountain were studied.The results indicated that the media for inducing shoot clusters was WPM+ZT 2.0 mg/L;the medium for subculture multiplication culture was modified MS+ZT 0.5 mg/L+IBA 0.2 mg/L,and the coefficient of multiplication was 5.3 after culture for 30 days;the medium for the rooting was 1/4 MS+IBA 0.5 mg/L with rooting rate of over 80% after culture for 50 days.Practices transplanting seedling was to tissue culture in sand 20 days,and then transplanting seedlings to the turfy soil: humus soil 1∶2,and survival rate in nursery was up to 78%. |
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| Keywords: | Vaccinium uliginosum superior individual in vitro culture rapid propagation |
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