| 番茄SRAP-PCR反应体系建立与优化 |
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| 引用本文： | 郝凤,于铁峰,雷银川.番茄SRAP-PCR反应体系建立与优化[J].北方园艺,2012(3):107-109. |
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| 作者姓名： | 郝凤 于铁峰 雷银川 |
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| 作者单位： | 甘肃省航天育种工程技术研究中心,甘肃天水,741030 |
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| 基金项目： | 国家农业科技成果转化资金资助项目 |
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| 摘 要： | 利用正交实验设计L16(45)对番茄SRAP-PCR反应体系的5个因素(Mg2+、dNTPs、引物、Taq DNA聚合酶和模板DNA)在4个水平上进行优化试验研究。结果表明:各因素水平变化对反应体系影响的大小依次为:Mg2+dNTPs引物Taq DNA聚合酶模板DNA;建立的番茄SRAP-PCR最佳体系(25μL)为:Mg2+2.5mmol/L、Taq DNA聚合酶0.5U、dNTPs0.25mmol/L、引物0.4μmol/L、模板DNA 80ng。
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| 关 键 词： | 番茄 SRAP 正交设计 体系优化 |
Optimization for SRAP-PCR System of Tomato Based on Orthogonal Design |
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| HAO Feng
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YU Tie-feng
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LEI Yin-chuan.Optimization for SRAP-PCR System of Tomato Based on Orthogonal Design[J].Northern Horticulture,2012(3):107-109. |
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| Authors: | HAO Feng YU Tie-feng LEI Yin-chuan |
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| Institution: | (Space Breeding Engineering Research Center of Gansu Province,Tianshui,Gansu 741030) |
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| Abstract: | The SRAP-PCR system of Tomato was optimized by the orthogonal design which was in four levels of five factors(Mg2+,Taq DNA polymerase,dNTPs,Primer,and DNA template).The results showed that the order of each factor in different levels affected the result of PCR was:Mg2+>dNTPs>primer>Taq DNA polymerase>DNA template;the optimum SRAP-PCR system(25 μL) for tomato included Mg2+ 2.5 mmol/L,Taq DNA polymerase 0.5 U,dNTPs 0.25 mmol/L,Primer 0.4 μmol/L and DNA template 80 ng. |
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| Keywords: | tomato SRAP orthogonal design system optimization |
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