| 内生真菌对不同地理种群中华羊茅生长及营养品质的影响 |
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| 引用本文： | 王凯,王伟林,王豪邦,裴天悦,董鑫,蔺伟虎,田沛.内生真菌对不同地理种群中华羊茅生长及营养品质的影响[J].草业科学,2020(3):522-531. |
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| 作者姓名： | 王凯 王伟林 王豪邦 裴天悦 董鑫 蔺伟虎 田沛 |
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| 作者单位： | 兰州大学草地农业生态系统国家重点实验室/兰州大学农业农村部草牧业创新重点实验室/兰州大学草地农业科技学院 |
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| 基金项目： | 国家自然科学基金(31502001);国家基础研究发展规划“973”(2014CB138702)。 |
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| 摘 要： | 本研究以分布于青海、四川的6个中华羊茅(Festuca sinensis)地理种群及其Epichlo?内生真菌为研究对象,分别构建了带Epichlo?内生真菌(E+)和不带内生真菌的(E?)种群,比较了其在温室盆栽条件下的生长状况(分蘖、茎粗、叶宽、株高、鲜重和干重)和营养成分(粗脂肪、粗蛋白、粗灰分和叶片含水量),旨在探明内生真菌和寄主基因型对中华羊茅生长和营养品质的影响。结果表明:1)内生真菌对部分地理种群中华羊茅的株高、分蘖、茎粗、鲜重、粗脂肪(ether extract,EE)、粗蛋白(crude protein,CP)和叶片含水量有显著影响(P<0.05),且在不同地理种群中表现不一致。种群84 E+植株株高显著高于E?植株(P<0.05),种群41、57和141 E+植株粗脂肪含量显著高于相对应的E?植株(P<0.05),种群41、99和141 E+植株粗蛋白含量显著高于相对应的E?植株(P<0.05),种群99 E+植株叶片含水量显著高于E?植株(P<0.05);种群57 E+植株分蘖数显著低于E?植株(P<0.05),种群99 E+植株茎粗显著低于E?植株(P<0.05),种群57和99 E+植株株高显著低于相对应的E?植株(P<0.05),种群57 E+植株粗蛋白含量显著低于E?植株(P<0.05)。2)不同地理种群的E+和E?植株分蘖、茎粗、株高、鲜重和粗脂肪、粗蛋白含量有显著差异(P<0.05),且各种群表现不尽相同。6个地理种群E?植株,种群57的分蘖数显著高于种群84和99(P<0.05),种群57的粗蛋白含量显著高于其他5个地理种群(P<0.05),种群111的株高显著高于种群84和99(P<0.05),种群111的粗脂肪含量显著高于种群41和99(P<0.05);6个地理种群E+植株,种群111的分蘖数和茎粗均显著高于其他5个种群(P<0.05),种群141的鲜重和干重均显著高于种群41和84(P<0.05),种群111和141的粗脂肪含量均显著高于种群41、84和99(P<0.05);综合分析生长和营养指标,6个地理种群E?植株,种群57和111表现最好,6个地理种群E+植株,种群111和141表现最好。综上,内生真菌促进了部分地理种群(84)植株的生长,提高了部分地理种群(41和141)的营养品质,抑制了部分地理种群(99)植株的生长,降低了部分地理种群(57)的营养品质;寄主基因型对中华羊茅共生体的生长和营养品质显著影响(P<0.05)。
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| 关 键 词： | 中华羊茅 内生真菌 地理种群 生长 粗蛋白 粗脂肪 |
Effects of Epichloe endophyte on the growth and nutritional quality of different geographic populations of Festuca sinensis |
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| WANG Kai,WANG Weilin,WANG Haobang,PEI Tianyue,DONG Xin,LIN Weihu,TIAN Pei.Effects of Epichloe endophyte on the growth and nutritional quality of different geographic populations of Festuca sinensis[J].Pratacultural Science,2020(3):522-531. |
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| Authors: | WANG Kai WANG Weilin WANG Haobang PEI Tianyue DONG Xin LIN Weihu TIAN Pei |
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| Institution: | (State Key Laboratory of Grassland Agro-ecosystems/Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs/College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,Gansu,China) |
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| Abstract: | To investigate the effects of Epichloeendophyte on the growth and nutritional quality of different ecotypes of Festuca sinensis,six ecotypes of endophyte-infected(E+)and endophyte-free(E?)Festuca sinensis were established.The plant seeds were obtained from Qinghai and Sichuan provinces and compared for their growth(tiller,stem diameter,leaf width,plant height,fresh weight,and dry weight)and nutritional quality(crude fat,crude protein,crude ash,and moisture content)under pot cultures in a greenhouse.The results are as follows:1)Epichloeendophyte had a significant impact on the height,tiller,stem diameter,fresh weight,crude fat,crude protein,and moisture content of ecotypes(P<0.05),and these effects were different for different ecotypes.The height of ecotype 84 E+was significantly higher than that of E?plants(P<0.05),while the crude fat content of ecotypes 41 E+,57 E+,and 141 E+was significantly higher than that of the corresponding E?plants(P<0.05).The crude protein content of ecotypes 41 E+,99 E+,and 141 E+plant was significantly higher than that of the corresponding E?plants(P<0.05),and the moisture content of the ecotype 99 E+was significantly higher than that of E?plants(P<0.05).Moreover,tillering number was significantly lower for ecotype 57 E+than that for E?plants(P<0.05),while the stem diameter of ecotype 99 E+was significantly lower than that of E?plants(P<0.05).The height of ecotypes 57 E+and 99 E+was significantly lower than that of the corresponding E?plants(P<0.05),and the crude protein content of ecotype 57 E+was significantly lower than that of E?plant(P<0.05).2)Significant differences were reported in tiller,stem diameter,plant height,fresh weight,crude fat,and crude protein content among different geographical populations,while the performance of various groups was different(P<0.05).Among the six endophyte-free(E?)F.sinensis populations,the tiller number of ecotype 57 was significantly higher than that of the ecotypes 84 and 99(P<0.05),and the crude protein content was significantly higher for ecotype 57 than that for the other five ecotypes(P<0.05).The height of ecotype 111 was significantly higher than that of ecotypes 84 and 99(P<0.05),and the crude fat content of ecotype 111 was significantly higher than that of the ecotypes 41 and 99(P<0.05).Among the six endophyte-infected(E+)F.sinensis populations,ecotype 111 had significantly higher tiller number and stem diameter than the other five ecotypes(P<0.05).Both fresh weight and dry weight of ecotype 141 were significantly higher than those of ecotypes 41 and 84(P<0.05),while the crude fat content of ecotypes 111 and 141 was significantly higher than that of ecotypes 41,84,and 99(P<0.05).Comprehensive analysis of growth and nutrition indicators showed that ecotypes 57 and 111 were the best among the six endophyte-free(E?)F.sinensis populations,while ecotypes 111 and 141 were the best among the six endophyte-infected(E+)F.sinensis populations.In summary,the endophytic fungi promoted growth(84)and improved nutritional quality(41 and 141)or restrained growth(99)and reduced nutritional quality(57).Thus,the host genotype had a significant impact on the growth and nutritional quality of Epichloeendophyte-F.sinensis symbiont(P<0.05). |
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| Keywords: | Festuca sinensis Epichloe endophyte geographical population growth crude protein crude fat |
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