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草甘膦及其代谢物氨甲基膦酸在茶树体中的分布研究
引用本文:郭永春,陈金发,赵峰,王淑燕,王鹏杰,周鹏,欧阳立群,金珊,叶乃兴.草甘膦及其代谢物氨甲基膦酸在茶树体中的分布研究[J].茶叶科学,2020,40(4):510-518.
作者姓名:郭永春  陈金发  赵峰  王淑燕  王鹏杰  周鹏  欧阳立群  金珊  叶乃兴
作者单位:1.福建农林大学园艺学院/茶学福建省高校重点实验室,福建 福州 350002;2.国家茶叶质量安全工程技术研究中心,福建 安溪362400;3.福建中医药大学药学院,福建 福州 350122;4.福建省产品质量检验研究院,福建 福州 350002
基金项目:福建农林大学茶产业链科技创新与服务体系建设项目(2020-01)、国家茶叶质量安全工程技术研究中心开放课题(2018NTQS0301)、福建农林大学园艺学院优秀硕士学位论文资助基金(2019S01)
摘    要:为了研究草甘膦在茶树中的转运和代谢,通过向幼龄茶树和成年茶树定量喷施不同剂量的草甘膦(幼龄:0.9 g·m-2;成年:1.5、4.5 g·m-2和15 g·m-2),并在喷施后的1、4、7、15 d和40 d取样,测定茶树各部位草甘膦及其主要代谢物氨甲基膦酸的含量,以探究草甘膦及其主要代谢物在茶树中的分布规律。结果表明,茶树根部喷施草甘膦后,茶树中的草甘膦含量由高至低依次为根、叶和茎;而氨甲基膦酸主要积累于茶树根部,幼龄茶树茎和叶中也可检测到氨甲基膦酸(0~0.33 mg·kg-1),成年茶树茎和叶未检测到氨甲基膦酸。施药40 d后,幼龄茶树在0.9 g·m-2施药剂量下,根、茎和叶中未检测到草甘膦和氨甲基膦酸;成年茶树在1.5~15 g·m-2施药剂量下,根中仍检测到草甘膦和氨甲基膦酸残留,分别为2.26~26.73 mg·kg-1和0.21~2.59 mg·kg-1,茎和叶中草甘膦残留量较低,为0~0.29 mg·kg-1。通过田间模拟试验,初步探明了草甘膦被茶树内吸后在不同部位的转运、代谢和富集规律,可为茶园草甘膦的科学管控及风险评价提供参考。

关 键 词:茶树  草甘膦  氨甲基膦酸  残留  分布  
收稿时间:2020-03-01

Study on the Distribution of Glyphosate and Its Metabolite Aminomethylphosphonic Acid in Camellia Sinensis
GUO Yongchun,CHEN Jinfa,ZHAO Feng,WANG Shuyan,WANG Pengjie,ZHOU Peng,OUYANG Liqun,JIN Shan,YE Naixing.Study on the Distribution of Glyphosate and Its Metabolite Aminomethylphosphonic Acid in Camellia Sinensis[J].Journal of Tea Science,2020,40(4):510-518.
Authors:GUO Yongchun  CHEN Jinfa  ZHAO Feng  WANG Shuyan  WANG Pengjie  ZHOU Peng  OUYANG Liqun  JIN Shan  YE Naixing
Institution:1. College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian province, Fuzhou 350002, China;2. National Engineering Research Center for Tea Quality-and-Safety, Anxi 362400, China;3. School of Pharmacy, Fujian University of Chinese Medicine, Fuzhou 350122, China;4. Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China
Abstract:To study the transport and metabolism of glyphosate, different doses of glyphosate were quantitatively sprayed into young and adult tea plants (Young: 0.9 g·m-2. Adult: 1.5 g·m-2, 4.5 g·m-2 and 15 g·m-2) and samples were collected at different times (1, 4, 7, 15 d and 40 d) to determine the contents of glyphosate and its main metabolite aminomethylphosphonic acid in various parts of tea plants. The results show that the order of glyphosate content in tea plants from high to low was root, leaf and stem after spraying glyphosate on the root of tea plant. Aminomethylphosphonic acid was mainly accumulated in the roots of tea plants, and it could also be detected in the stems and leaves of young tea plants (0-0.33 mg·kg-1). However, no aminomethylphosphonic acid was detected in the stems and leaves of adult tea plants. After 40 d of application, glyphosate and aminomethylphosphonic acid were not detected in the roots, stems and leaves of young tea plants at a dose of 0.9 g·m-2. However, they were still detected in the roots of adult tea plants at a dose of 1.5-15 g·m-2 (glyphosate: 2.26-26.73 mg·kg-1, aminomethylphosphonic acid: 0.21-2.59 mg·kg-1). Glyphosate residues in both stem and leaves of adult plants were low (0-0.29 mg·kg-1). In this study, through field simulation experiments, the transfer, metabolism and enrichment rules of glyphosate in tea plants after being ingested were analyzed, which could provide a reference for scientific control and risk assessment of glyphosate in tea gardens.
Keywords:Camellia sinensis  glyphosate  aminomethylphosphonic acid  residue  distribution  
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