| 油松胚性愈伤组织成熟培养的影响因素 |
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| 引用本文： | 李亦轩,江帅菲,董明亮,齐帅征,张金凤,Lisheng Kong.油松胚性愈伤组织成熟培养的影响因素[J].东北林业大学学报,2020(4):20-23,49. |
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| 作者姓名： | 李亦轩 江帅菲 董明亮 齐帅征 张金凤 Lisheng Kong |
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| 作者单位： | 林木育种国家工程实验室（北京林业大学）;Department of Biology |
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| 基金项目： | 国家重点研发计划(2017YFD0600501);中央高校基本科研业务费专项资金(2015ZCQ-SW-02)。 |
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| 摘 要： | 以油松的胚性愈伤组织为材料,利用单因素试验设计探究ABA质量浓度、PEG质量浓度、植物凝胶质量浓度、糖的种类和其质量浓度对油松体细胞胚成熟率的影响;在此基础上采用正交试验筛选出最佳培养条件。单因素试验结果表明,成熟培养基中ABA质量浓度14~18 mg·L^-1、PEG质量浓度50~100 g·L^-1、植物凝胶质量浓度2.5~5.0 g·L^-1、麦芽糖和蔗糖质量浓度组合(15+25)g·L^-1时,体细胞胚的成熟效率较高;正交试验结果表明,成熟培养基中ABA质量浓度16 mg·L^-1、PEG质量浓度75 g·L^-1、植物凝胶质量浓度5 g·L^-1、麦芽糖和蔗糖质量浓度(10+30)g·L^-1时,油松体细胞胚的成熟效率提高了0.8倍。
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| 关 键 词： | 油松 体胚发生 胚性愈伤组织 体细胞胚 |
Maturational Factors in Mature Culture of Embryogenic Callus of Pinus tabulaeformis |
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| Institution: | (National Engineering Laboratory for Tree Breeding,Beijing Forestry University,Beijing 100083,China;Department of Biology,University of Victoria,Victoria,BC,Canada) |
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| Abstract: | With embryogenic callus of Pinus tabulaeformis,we performed a serious of single factor experiments and calculated the maturation rate of embryogenic callus under the treatments of different concentrations of ABA,PEG,Phytagel,type and concentration of sugar.Orthogonal experiment was then conducted to screen out the best maturation cultural medium.Results of the single factor experiments showed that maturation rate was relatively high under 14-18 mg·L^-1 ABA,50-100 g·L^-1 PEG,2.5-5.0 g·L^-1 phytagel,and 15+25 g·L^-1 maltose+sucrose were added to the mature medium.By the orthogonal design,adding 16 mg·L^-1 ABA,75 g·L^-1 PEG,5 g·L^-1 phytagel and 10+30 g·L^-1 maltose+sucrose to the mature medium,the maturation rate was increased by 0.8 times. |
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| Keywords: | Pinus tabulaeformis Somatic embryogenesis Embryogenic callus Somatic embryo |
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