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| 桉叶抗氧化物的提取与抗氧化性质研究 | |
| 引用本文： | 陈海,田宏,肖苏尧,曹庸,黄景晟,王俊亮.桉叶抗氧化物的提取与抗氧化性质研究[J].湖南林业科技,2009,36(2):25-29. |
| 作者姓名： | 陈海 田宏 肖苏尧 曹庸 黄景晟 王俊亮 |
| 作者单位： | 1. 茂名市林业局,广东,茂名,525000 2. 湖南农业大学,湖南,长沙,410128 3. 华南农业大学,广东,广州,510642 |
| 摘 要： | 以广林9号桉叶粉为材料，通过单因素和正交实验，确定最佳提取条件为50％乙醇、料液比1：20、50℃提取1h；抗氧化稳定性研究表明：光照、空气和温度对提取物抗氧化活性均有较大的影响，而pH值对其影响较小；对酚类物质的含量进行测定，表明提取物中酚类物质的含量与抗氧化效果成正相关；以有机溶剂对上述提取物进行萃取，抗氧化效果为正丁醇萃取物〉乙酸乙酯萃取物〉水萃取物〉石油醚萃取物；然后比较了不同浓度乙醇作为洗脱剂对正丁醇萃取物分离的影响，以50％和70％乙醇洗脱成分活性最强。 |
| 关 键 词： | 桉叶 提取物 抗氧化 DPPH.自由基 清除率 |
Research on extraction of antioxidants and antioxidant properties of Eucalyptus leaves |
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| CHEN Hai,TIAN hong,XIAO Suyao,CAO Yong,HUANG Jingsheng,WANG Junliang.Research on extraction of antioxidants and antioxidant properties of Eucalyptus leaves[J].Hunan Forestry Science & Technology,2009,36(2):25-29. | |
| Authors: | CHEN Hai TIAN hong XIAO Suyao CAO Yong HUANG Jingsheng WANG Junliang |
| Institution: | 1.Forestry Bureau of Maoming City;Maoming 525000;China;2.Hunan Agricultural University;Changsha 410128;3.South China Agricultural University;Guangzhou 510642;China |
| Abstract: | Through single factor and orthogonal experiment with Eucalyptus GL9 leaves powder as material. The result showed that： （1） Optimal extracting parameters of antioxidants from Eucalyptus leaves as followed： 50% ethanol, solidliquid ratio 1：20 with the extraction time of lh under temperature 50℃ ; （2） Study on the antioxidant stability showed that light, air and temperature had great impact on the antioxidant activity of extracts but pH has less impact; （ 3 ） Test of phenolic substances content showed the content of phenolic substances from extract and antioxidant effect is positively correlated; （4） Using organic solvent for the extraction of the extract, we got the order of antioxidant effect as followed： n - butanol extract 〉 ethyl acetate extract 〉 water extract 〉 petroleum ether extract; （5） The 50% and 70% ethanol elution parts from Polyamide Chromatography were proved to have better antioxidant capacity than other parts. |
| Keywords: | Eucalyptus leaf extract antioxldant DPPH radical clearance rate |
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