Molecular cloning and expression of salmon pituitary hormones |
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| Authors: | Choy L Hew Khiet Ye Trinh Shao Ju Du Shiduo Song |
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| Institution: | (1) Research Institute, Hospital for Sick Children, Toronto and Depts. of Clinical Biochemistry and Biochemistry, University of Toronto, Toronto, Ontario;(2) Department of Clinical Biochemistry, University of Toronto, 100 College Street, Toronto, Ontario, Canada, M5G 1L5 |
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| Abstract: | A cDNA library was prepared from chinook salmon pituitaries. Growth hormone (GH), prolactin (PRL) and the β subunit of gonadotropin
(GTH) genes were screened using synthetic oligonucleotides as probes. Full size cDNA clones coding for these polypeptide hormones
were isolated and characterized. The cDNA sequences for PRL and βGTH have been reported earlier from our laboratories. The
cDNA clone for GH contains 1148 bp and codes for a preGH of 210 amino acids. The chinook salmon GH, reported in the present
investigation, differs from chum salmon GH in only 1 amino acid, and from coho salmon GH in 5 amino acids. Plasmids containing
modified nucleotide sequences coding for GH, PRL and βGTH were constructed individually into an expression vector using the
heat-inducible λ pL promotor. Mature PRL, GH and unglycosylated βGTH were expressed in the bacteria at elevated temperature. |
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| Keywords: | growth hormone prolactin gonadotropin cDNA cloning Lamda promotor synthetic hormones |
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