|
|||||
|
|
| 几丁质酶基因和β-1,3-葡聚糖酶基因克隆微生物表达和抑菌效果研究 | |
| 引用本文： | 刘玲玲,张金文,王旺田,陈正华.几丁质酶基因和β-1,3-葡聚糖酶基因克隆微生物表达和抑菌效果研究[J].甘肃农业大学学报,2004,39(3):261-265. |
| 作者姓名： | 刘玲玲 张金文 王旺田 陈正华 |
| 作者单位： | 1. 甘肃省作物改良与种质创新重点实验室,甘肃农业大学,甘肃,兰州,730070 2. 甘肃省作物改良与种质创新重点实验室,甘肃农业大学,甘肃,兰州,730070;中国科学院西北高原生物研究所,青海,西宁,810080;甘肃亚盛集团博士后科研工作站,甘肃,兰州,730035 3. 甘肃亚盛集团博士后科研工作站,甘肃,兰州,730035 |
| 基金项目： | 甘肃省自然科学基金(编号:ZS991-A21-029-N) |
| 摘 要： | 采用PCR扩增的方法,扩增获得几丁质酶和β-1,3-葡聚糖酶的基因,分别将其基因克隆到原核表达载体pET28a(+)上,构建了原核表达载体pETChi和pETGlu。经IPTG诱导后几丁质酶基因和β-1,3-葡聚糖酶基因在大肠杆菌BL21中得到高效表达。通过对连孢(Alternaria alternate)的抑菌实验表明,这两基因的表达产物对此真菌有较强的抵抗能力。 |
| 关 键 词： | 几丁质酶 β-1 3-葡聚糖酶 原核表达 抑菌效果 |
| 文章编号： | 1003-4315(2004)03-0261-05 |
Studies on cloning and expression of the β-1,3-Glucanase and chitinase genes and their potential for fungal resistance |
|
| LIU Ling-ling,ZHANG Jin-wen,WANG Wang-tian,CHEN Zheng-hua.Studies on cloning and expression of the β-1,3-Glucanase and chitinase genes and their potential for fungal resistance[J].Journal of Gansu Agricultural University,2004,39(3):261-265. | |
| Authors: | LIU Ling-ling ZHANG Jin-wen WANG Wang-tian CHEN Zheng-hua |
| Abstract: | Chitinase and -1, 3-glucanase genes were amplified by PCR procedures and subcloned into an expression vector Pet-28a(+), the prokanyo expression vector of the both genes were constructed and named pETChi and pETGlu, respectively. A SDS-PAGE analysis indicated that chitinase and -1, 3-glucanase genes were expressed highly in E. coli BL21 after induced by IPTG. Challenged with Alternaria alternate showed that chitinase and -1, 3-glucanase genes had good resistance to the pathogenic fungus. |
| Keywords: | chitinase genes -1 3-Glucanase genes prokanyo expression resistance fungus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |