Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal |
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| Authors: | Hisao Yoshikawa Zhiliang Wu Kishor Pandey Basu Dev Pandey Jeevan Bahadur Sherchand Tetsuo Yanagi Hiroji Kanbara |
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| Institution: | 1. Institute of Parasitology, Vetsuisse Faculty, University of Bern, P.O. Box 8466, 3001 Bern, Switzerland;2. SAFOSO, Bremgartenstrasse 109A, 3012 Bern, Switzerland;3. Centre for Fish and Wildlife Health (FIWI), Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, P.O. Box 8466, 3001 Bern, Switzerland |
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| Abstract: | To investigate the possible transmission of Blastocystis organisms between local rhesus monkeys and children in Kathmandu, Nepal, we compared the subtype (ST) and sequence of Blastocystis isolates from children with gastrointestinal symptoms and local rhesus monkeys. Twenty and 10 Blastocystis isolates were established from 82 and 10 fecal samples obtained from children and monkeys, respectively. Subtype analysis with seven sequence-tagged site (STS) primers indicated that the prevalence of Blastocystis sp. ST1, ST2 and ST3 was 20%, 20% and 60% in the child isolates, respectively. In contrast to human isolates, ST3 was not found in monkey isolates and the prevalence of ST1 and ST2 was 50% and 70%, respectively, including three mixed STs1 and 2 and one isolate not amplified by any STS primers, respectively. Since Blastocystis sp. ST2 has been reported as the most dominant genotype in the survey of Blastocystis infection among the various monkey species, sequence comparison of the 150 bp variable region of the small subunit rRNA (SSU rRNA) gene was conducted among ST2 isolates of humans and monkeys. Sequence alignment of 24 clones developed from ST2 isolates of 4 humans and 4 monkeys showed three distinct subgroups, defined as ST2A, ST2B and ST2C. These three subgroups were shared between the child and monkey isolates. These results suggest that the local rhesus monkeys are a possible source of Blastocystis sp. ST2 infection of humans in Kathmandu. |
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| Keywords: | Blastocystis Human Monkey Subtype Small subunit rRNA Gene Phylogeny |
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