| 苎麻纤维发育相关的3个miRNA鉴定与表达分析 |
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| 引用本文： | 马永红,朱四元,严理,刘头明.苎麻纤维发育相关的3个miRNA鉴定与表达分析[J].中国麻业,2021(1):1-7. |
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| 作者姓名： | 马永红 朱四元 严理 刘头明 |
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| 作者单位： | 中国农业科学院麻类研究所 |
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| 基金项目： | 国家自然科学基金项目(31871678)。 |
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| 摘 要： | 研究以苎麻品种中苎1号为材料,开展其茎皮小RNA(miRNA)测序.结果显示,在苎麻茎皮中,共检测到378个miRNA,分析了4个管家基因在苎麻6个部位中的表达水平,发现18s在各组织中稳定表达.以18s基因作为内参基因,分析了3个候选miRNA(miR156、miR166和miR828)在苎麻6个部位中的表达谱,发现...
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| 关 键 词： | 苎麻 纤维发育 miRNA 表达分析 |
Identification and Expression Analysis of Ramie Fiber Developing-related miRNA |
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| MA Yonghong,ZHU Siyuan,YAN Li,LIU Touming.Identification and Expression Analysis of Ramie Fiber Developing-related miRNA[J].Plant Fibers and Products,2021(1):1-7. |
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| Authors: | MA Yonghong ZHU Siyuan YAN Li LIU Touming |
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| Institution: | (Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205,China) |
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| Abstract: | In this study,a small RNA(miRNA)sequencing was performed for the stem barks of the ramie variety Zhongzhu No.1.A total of 378 miRNAs was identified in the stem barks of ramie.In addition,four housekeeping genes were used for analyzing their expression levels in six tissues,and it was found that the 18 s gene displayed a steady expression in these investigated tissues.Subsequently,18 s gene were used as a reference gene to analyze the expression pattern of three miRNA,miR156,miR166 and miR828 in six tissues of ramie.The results revealed that the three miRNAs showed a higher expression level in the shoots and bast barks at the top stem than that in other tissues.And all three miRNAs showed differential expression between the barks at the top of stem and middle of stem,indicating they may have potential roles in the regulation of fiber growth.Bioinformatic prediction suggested that these 3 miRNAs targeted to regulate the function of homolog of ramie fiber developmental genes,including the MYB,NAC and HDZIP.This study provided an important basis for the study of miRNA function in the fiber development of ramie. |
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| Keywords: | ramie fiber development miRNA expression analysis |
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