| 山西一例牛病毒性腹泻病例病原分离鉴定及测序分析 |
| |
| 引用本文： | 肖望成,孙玉林,李蓉,曾维欢,李晓成,魏荣,代飞燕,吴发兴.山西一例牛病毒性腹泻病例病原分离鉴定及测序分析[J].中国奶牛,2021(2):28-32. |
| |
| 作者姓名： | 肖望成 孙玉林 李蓉 曾维欢 李晓成 魏荣 代飞燕 吴发兴 |
| |
| 作者单位： | 云南农业大学;中国动物卫生与流行病学中心;昆明市技术合同认定工作站 |
| |
| 基金项目： | 2020年农业财政专项——动物疫情监测与防治。 |
| |
| 摘 要： | 为查找引起山西某牛场疑似牛病毒性腹泻病例的病因,对送检的9份牛鼻腔棉拭子样品,经处理后进行了多病原PCR或RT-PCR检测、病原分离、特征性细胞病变观察、效价测定、RT-PCR鉴定及基因测序分析。结果显示:从9份样品中检出6份BVDV核酸阳性,IBRV、BRSV、BPIV3、支原体均阴性,病料上清接种MDBK细胞进行培养,传至F5代发现MDBK细胞出现特征性病变,表现为细胞变亮、圆缩、拉网并逐渐形成空泡,效价为10-5.42TCID50/0.1mL,BVDV RT-PCR鉴定阳性,将其命名为BVDV-SX2020株,测序发现其5’UTR与BVDV Oregon C24V等参考毒株同属BVDV基因1a亚型。结果证实该起病例由BVDV-1a毒株感染引起。
|
| 关 键 词： | 牛病毒性腹泻 PCR 分离鉴定 进化树 |
Isolation,Identification and Sequencing of a Case of Bovine Viral Diarrhea in Shanxi Province |
| |
| XIAO Wang-cheng,SUN Yu-lin,LI Rong,ZENG Wei-huan,LI Xiao-cheng,WEI Rong,DAI Fei-yan,WU Fa-xing.Isolation,Identification and Sequencing of a Case of Bovine Viral Diarrhea in Shanxi Province[J].China Dairy Cattle,2021(2):28-32. |
| |
| Authors: | XIAO Wang-cheng SUN Yu-lin LI Rong ZENG Wei-huan LI Xiao-cheng WEI Rong DAI Fei-yan WU Fa-xing |
| |
| Institution: | (Veterinary Medicine of Yunnan Agricultural University,Kunming 650201;China Animal Health and Epidemiology Center,Qingdao 266032;Technical Contract Recognition Workstation,Kunming 650021) |
| |
| Abstract: | In order to find the cause of a suspected bovine viral diarrhea case in a cattle farm in Shanxi,9 samples of bovine nasal swabs submitted for testing were processed for multi-pathogen PCR or RT-PCR detection,pathogen isolation,and characteristic cytopathy observation,titer determination,RT-PCR identification and gene sequencing analysis.The results showed that 6 out of 9 samples were positive for BVDV nucleic acid,and all negative for IBRV,BRSV,BPIV3,and Mycoplasma.The supernatant of the diseased material was inoculated with MDBK cells for culture,and passed to F5 generation,it was found that the MDBK cells showed characteristic lesions,which showed that the cells became bright,contracted,and netted,and gradually formed vacuoles.BVDV RT-PCR was positive and named it as BVDV-SX2020 strain.Sequencing revealed that its 5′UTR and BVDV Oregon C24 V and other reference strains belonged to subtype 1 a of BVDV gene.The results confirmed that the case was caused by BVDV1 a strain infection. |
| |
| Keywords: | Bovine viral diarrhea PCR Clementines Evolutionary tree |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
