Potential of a Fusarium eumartii culture filtrate on the screening for wilting resistance in potato |
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| Authors: | Grisela L Botta María P Dimarco Alicia L Melegari Marcelo A Huarte Carlos A Barassi |
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| Institution: | (1) Unidad Integrada Balcarce (INTA-UNMP), CC 276, 7620 Balcarce, Argentina |
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| Abstract: | Summary
Fusarium solani f.sp. eumartii Carp. Snyder and Hansen (Fusarium eumartii) is a soil inhabitant that induces the so-called Potato Wilt and Stem End Rot disease. Prior to wilting, the pathogen induces peculiar small bronze spots on the leaflets. Failure to isolate F. eumartii from infected leaflets suggests the involvement of a toxin in the disease. The fungus was grown in liquid Richard's medium and thereafter a filtrate was obtained dialyzing (MW cutoff 12,000–14,000) and sterilizing the culture by filtration (0.22  m). Potato leaves treated with both the pathogen or the filtrate showed symptoms of bronze spots and significantly higher electrolyte leakage when compared to controls. Tomato leaves showed neither bronze spots nor electrolyte leakage after plant inoculation with the pathogen or with the filtrate treatment. Both, the absence of visible symptoms and the lack of electrolyte leakage in tomato could be associated to a certain degree of host specificity of the F. eumartii filtrate towards potato. The filtrate also induced symptoms similar to infections by F. eumartii in adult plants and in vitro plantlets of cultivars Huinkul MAG and Kennebec. Callus responses to the filtrate were related to responses of the cultivars to the pathogen in greenhouse. These results show the potential of the culture filtrate of F. eumartii for use in screening for wilting resistance. |
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| Keywords: | Fusarium solani f sp eumartii culture filtrate electrolyte leakage potato wilting resistance toxin Solanum tuberosum |
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