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山核桃属2个树种叶片水浸提物的化感作用及其化感物质的含量比较
引用本文:张博,何开跃,郭丽君,翟敏.山核桃属2个树种叶片水浸提物的化感作用及其化感物质的含量比较[J].江苏林业科技,2014(1):1-6,16.
作者姓名:张博  何开跃  郭丽君  翟敏
作者单位:[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]南京绿宙薄壳山核桃科技有限公司,江苏南京211500
摘    要:以水浸提法提取薄壳山核桃和山核桃2种植物的叶片内含物,研究其对刺槐、萝卜和黑麦草种子萌发和幼苗生长的化感作用。结果表明:水浸提物明显抑制了3种受试植物种子的萌发及其幼苗的生长;生长7 d的幼苗体内,可溶性蛋白、SOD活性、POD活性降低,MDA含量升高。通过氯仿热回流及高效液相色谱法,提取、鉴定了2种植物叶片中的化感物质胡桃醌,并测定其含量。结果表明,薄壳山核桃叶片中所含有的胡桃醌含量高于山核桃。

关 键 词:山核桃属  水浸提物  化感作用  胡桃醌

Study on allelopathy of the leaves of two Carya species
ZHANG Bo,HE Kai-yue,GUO Li-jun,ZHAI Min.Study on allelopathy of the leaves of two Carya species[J].Journal of Jiangsu Forestry Science & Technology,2014(1):1-6,16.
Authors:ZHANG Bo  HE Kai-yue  GUO Li-jun  ZHAI Min
Institution:1 College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2. Nanjing Green Universe Pecan Science & Technology Co. ,Ltd. Nanjing 211500,China)
Abstract:The allelopathic effects of the water extract of Carya illinoensis (Wangench.) K. Koch and Carya cathayensis Sarg. leaves on the seed germination and seedling growth of Robinia pseudoacacia, Raphanus sativus, Lolium multiflorum were studied in this paper. The results indicated that their water extract had significant inhibitiory effect. The protein con- tent, the activities of SOD and POD in the seven days'seedlings were decreased, but malondiadehyde (MDA)in them was increased. Juglone was extracted from their leaves and its content was determined with chloroform thermal reflux and high performance Liquid chromatography ( HPLC ). Our result showed that the content of juglone in Carya illinoensis ( Wan- gench. ) K. Koch was higher than that in Carya cathayensis Sarg.
Keywords:Carya Nutt  Water extract  Allelopathic effect  Juglone
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