| 植物生长物质诱导芦荟叶片愈伤组织效果的研究 |
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| 引用本文： | 肖 波,廖尔华,胡开治,林茂祥.植物生长物质诱导芦荟叶片愈伤组织效果的研究[J].中国农学通报,2006,22(1):163-163. |
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| 作者姓名： | 肖 波 廖尔华 胡开治 林茂祥 |
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| 作者单位： | 1. 重庆市药物种植研究所,重庆,南川,408435
2. 四川农业大学资源与环境学院,四川,雅安,625014 |
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| 摘 要： | 采用正交设计的方法，考察了6-BA、NAA、2.4-D、IBA这四种常用生长调节物质对中华芦荟叶片愈伤组织的诱导效果。结果表明：在MS基本培养基中，这四种生长物质对芦荟叶片愈伤组织的诱导均具极显著效果，其诱导效果大小表现为6-BA>IBA>2.4-D>NAA；在多种不同的生长物质配比中，以6-BA4.0mg/L+NAA0.25mg/L+2.4-D3.0mg/L+IBA0.5mg/L对愈伤组织的诱导效果最好。
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| 关 键 词： | 芦荟 生长物质 愈伤组织诱导 正交设计 |
| 收稿时间： | 2005-08-08 |
| 修稿时间： | 2005-08-082005-09-02 |
Study on Callus induction of Aloe Leaves with Plant Growth Substances |
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| Xiao Bo,Liao Erhu,Hu Kaizhi,Lin Maoxiang.Study on Callus induction of Aloe Leaves with Plant Growth Substances[J].Chinese Agricultural Science Bulletin,2006,22(1):163-163. |
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| Authors: | Xiao Bo Liao Erhu Hu Kaizhi Lin Maoxiang |
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| Institution: | (1Chongqing Institute of Medicinal Plant Cultivation, Nanchuan Chongqing 408435; 2Colledge of Resources And Environment, Sichuan Agricultural University, Ya’an Sichuan 625014) |
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| Abstract: | The effects of 6-BA, NAA, 2.4-D, and IBA on the leave callus of Aloe were studied by using L9(34) orhogonal design. The variance analysis of the experimental results showed that the actions of the four factors were all extremely remarkable in MS medium. Among the four factors, 6-BA was the most important one for the callus induction of Aloe leaves, followed by IBA, then 2.4-D, and NAA was the weakest .The best combination of plant growth substances for callus induction was 6-BA4.0mg/L NAA0.25mg/L 2.4-D3.0mg/L IBA0.5mg/L. |
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| Keywords: | Aloe Plant growth substances Callus induction Orthogonal design |
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