| 硫素营养水平对大豆籽粒OAS-TL表达的影响 |
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| 引用本文： | 马淑梅,刘丽君,孙聪姝,董守坤,祖伟.硫素营养水平对大豆籽粒OAS-TL表达的影响[J].东北农业大学学报,2010,41(1). |
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| 作者姓名： | 马淑梅 刘丽君 孙聪姝 董守坤 祖伟 |
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| 作者单位： | 东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030;东北农业大学农学院,哈尔滨,150030 |
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| 基金项目： | 国家“十一五”科技支撑资助项目(2006BAD21B01-5,2006BAD21B01-8) |
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| 摘 要： | 大豆氧乙酰丝氨酸(硫醇)裂解酶(OAS-TL)催化半胱氨酸合成,是硫素代谢的关键酶。试验以东农46(高油型)和黑农35(高蛋白型)为材料,检测鼓粒期后大豆籽粒OAS-TL在3个不同硫水平(0、0.02、0.08g·kg-1土)下的生理特性,鉴定克隆了该酶基因并分析了其调控表达情况。结果表明,①OAS-TL活性随生育进程推进持续下降,2个品种籽粒OAS-TL活性表现为S20高于S0和S80处理;鼓粒前期施硫效果大于后期;②通过同源克隆获得一个编码OAS-TL基因的cDNA序列,全长1059bp,ORF长855bp,编码34.2ku的蛋白质;③半定量RT-PCR显示OAS-TL稳定态mRNA在籽粒发育早期表达较强,且处理间差异明显,发育后期表达水平逐渐降低。不同硫素处理间OAS-TL的表达有差异,S20上调表达高于S0和S80。
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| 关 键 词： | 大豆 氧乙酰丝氨酸(硫醇)裂解酶(OAS-TL) 硫 |
Effect of expression OAS-TL on soybean seeds under sulfur regulation |
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| MA Shumei,LIU Lijun,SUN Congshu,DONG Shoukun,ZU Wei.Effect of expression OAS-TL on soybean seeds under sulfur regulation[J].Journal of Northeast Agricultural University,2010,41(1). |
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| Authors: | MA Shumei LIU Lijun SUN Congshu DONG Shoukun ZU Wei |
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| Institution: | College of Agriculture;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | The objective of this study was to study and characterize O-acetyl-L-serine(thiol)-lyase (OAS-TL) which is a key enzyme in the pathway of sulfur metabolism that catalyzes the last step in the production of cysteine.Two cultivars, Dongnong46 (high-oil), Heinong35 (high-protein) were tested in this research by means of pots experiments with three sulfur application levels(0,0.02, 0.08 g·kg~(-1) soil) to study the effects of sulfur on the seeds of soybeans at the stage of drum particle. And than, cloned and characterized the expression OAS-TL. The results showed that:① The activity OAS-TL were higher at the early stage of seeds development and than declined along with the seeds maturity process. The treatment of S_(20) showed higher activity than those of S_0 and S_(80); The effect of each sulfur treatment showed more different at the early stage than the late stage;② A full-length cDNA clone encoding OAS-TL was obtained by homology cloning. The nucleotide sequenced covers 1 059 bp and nucleotides analysis of the cDNA revealed a single region of ORF of 855 bp encoding a 34.2 ku protein; ③RT-PCR analysis revealed that the OAS-TL mRNA was abundant and showed significant difference under each treatment at the early stage of seed development and than declined along with the seeds matudty process. The OAS-TL mRNA were different at each of the treatment, totally, the mRNA level of the treatment of S_(20) was more higher than those of S_0 and S_(80). |
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| Keywords: | soybean O-acetyl-L-sedne(thiol)-lyase(OAS-TL) sulfur |
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