| 鹅源性成分PCR检测方法的建立 |
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| 引用本文： | 汪燕玲,宗卉,阮周曦,张利平.鹅源性成分PCR检测方法的建立[J].安徽农业科学,2009,37(24):11432-11434. |
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| 作者姓名： | 汪燕玲 宗卉 阮周曦 张利平 |
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| 作者单位： | 甘肃农业大学动物科学技术学院,甘肃兰州,730070;深圳出入境检验检疫局动植中心,广州深圳,518010 |
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| 基金项目： | 深圳出入境检验检疫局资助项目 |
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| 摘 要： | 目的]建立一种检测鹅源性成分的PCR方法。方法]以15种动物的DNA为模板，利用鹅的特异性引物进行PCR反应，PCR产物用1．4％的琼脂糖凝胶电泳检测并测序，检测引物的特异性和敏感性。结果]通过PCR反应从鹅样品中扩增到了304bp的目的片段，其他物种火鸡、鸭、鸽鹑、鸵鸟、鸽子、鸡、马、牛、羊、猪、狗、驴、猫、鱼和空白对照则无目的片段，表明该引物的特异性良好。鹅组织PCR产物的核苷酸序列与GenBank中检索到的相应序列基本符合，相似性为98％；PCR检测方法的检测灵敏度为0．01％。结论]该PCR检测方法特异性强、准确性好、灵敏度高、可重复性好，为明确食品与饲料的成分和来源，预防禽流感的传播提供了有效的分子生物学捡测方法.
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| 关 键 词： | 鹅 线粒体DNA 动物源性成分 聚合酶链式反应 |
Establishment of PCR Detections for Goose-derived Components |
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| WANG Yan-ling et al.Establishment of PCR Detections for Goose-derived Components[J].Journal of Anhui Agricultural Sciences,2009,37(24):11432-11434. |
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| Authors: | WANG Yan-ling |
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| Institution: | WANG Yan-ling et al (Institute of Animal Science , Technology,Gansu Agricultural University,Lanzhou,Gansu 730070) |
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| Abstract: | Objective] The purpose was to establish a PCR method for detecting goose-derived components.Method] With DNAs from 15 animals as templates,PCR reactions were carried out by using specific primers of goose.The PCR products were detected by 1.4% agarose gel electrophoresis and sequenced to detect the specificity and sensitivity of primers.Result] The target fragment with length as 304 bp was amplified from goose samples through PCR reaction.There was no target fragment in other species such as turkeys,duck... |
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| Keywords: | Goose Mitochondrial DNA Animal-derived ingredients Polymerase chain reaction |
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