| 马尾松大配子体的SSR多样性及其遗传作图策略研究 |
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| 引用本文： | 蔡娟娟,季孔庶.马尾松大配子体的SSR多样性及其遗传作图策略研究[J].分子植物育种,2009,7(5):934-940. |
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| 作者姓名： | 蔡娟娟 季孔庶 |
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| 作者单位： | 林木遗传与生物技术省部共建教育部重点实验室,南京林业大学,南京,210037 |
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| 基金项目： | 十一五国家科技支撑项目 |
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| 摘 要： | 本研究利用马尾松单株160粒种子的胚乳（大配子体）和235对微卫星（SSR）引物构建马尾松遗传图谱。经过筛选得到30对具有多态性的引物,产生97个分离位点,平均1.62位点/引物,偏分离率为11.34%。利用FsLinkageMap2.0软件对其进行连锁关系分析,74个标记分布在11个连锁群,有23个标记未连锁到任何连锁群上。该图谱覆盖的基因组总长度为927.91cM,最大连锁群图距为359.89cM,最小连锁群图距为20.44cM。平均每个连锁群的长度为84.36cM,标记间最大间距为28.2cM,最小间距为0,标记平均距离为12.54cM。估算的马尾松基因组大小为2541.21cM,基因组覆盖度为36.5%。利用马尾松大配子体构建该树种较饱和的遗传图谱,大约需要超过1250个标记,并应积极尝试AFLP和SRAP等标记;群体数目应超过200个;同时应开发适于半同胞群体的作图软件。
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| 关 键 词： | 马尾松 大配子体 微卫星（SSR） 遗传图谱 |
Diversity of SSR Markers and Strategy of Genetic Linkage Map Making with Masson's Pine Megagametophytes |
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| Cai Juanjuan Ji Kongshu The Key Laboratory of Forest Genetics & Biotechnology,Ministry of Education,Nanjing Forestry University,Nanjing.Diversity of SSR Markers and Strategy of Genetic Linkage Map Making with Masson's Pine Megagametophytes[J].Molecular Plant Breeding,2009,7(5):934-940. |
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| Authors: | Cai Juanjuan Ji Kongshu The Key Laboratory of Forest Genetics & Biotechnology Ministry of Education Nanjing Forestry University Nanjing |
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| Institution: | Cai Juanjuan Ji Kongshu The Key Laboratory of Forest Genetics & Biotechnology,Ministry of Education,Nanjing Forestry University,Nanjing,210037 |
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| Abstract: | A number of 235 pairs primers was screened for simple sequence repeat markers within a sample of 160 megagametophyte DNAs from a Masson's pine(Pinus massoniana Lamb) to construct the molecular linkage map.97 separated loci were gained by using 30 pairs of screening primers,the average loci/primer was 1.62.The distorted segregation rate was 11.34%.Based on the 97 loci,the genetic linkage map of 74 loci was constructed by the software FsLinkageMap2.0.They were distributed evenly into 11 linkage groups while 2... |
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| Keywords: | Pine(Pinus massoniana Lamb) Megagametophyte Microsatellite(SSR) Genetic linkage map |
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