| 小尾寒羊雌激素受体基因外显子4的克隆与序列分析 |
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| 引用本文： | 贾立华,储明星,陈宏权,方丽.小尾寒羊雌激素受体基因外显子4的克隆与序列分析[J].中国畜牧兽医,2008,35(2):60-63. |
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| 作者姓名： | 贾立华 储明星 陈宏权 方丽 |
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| 作者单位： | 1.安徽农业大学动物科技学院，合肥 230036； 2.中国农业科学院北京畜牧兽医研究所，中国农业科学院家养动物遗传资源与种质创新重点开放实验室，北京 100094 |
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| 基金项目： | 国家自然科学基金项目(30300248)，北京市自然科学基金项目(5042017)，中国农业科学院北京畜牧兽医研究所基本科研业务费专项(ywf-rc-1)资助 |
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| 摘 要： | 根据GenBank发表的人、鸡、大鼠雌激素受体(estrogen receptor，ESR)基因外显子4的序列设计1对引物，采用PCR 技术扩增出小尾寒羊ESR基因外显子4的DNA片段，将该片段克隆到pGEM-T Easy 质粒中，重组质粒用PCR 扩增进行阳性克隆鉴定，然后测定其核苷酸序列并推导氨基酸序列，同时将测定的小尾寒羊ESR基因外显子4序列与人、牛、猪、大鼠、鸡的外显子4序列进行比较。结果表明：克隆测序所得的核苷酸和翻译后的氨基酸序列与人、牛、猪、大鼠、鸡相比，同源性分别为77.68%～97.28%和71.82%～98.18%，显示了很强的保守性；小尾寒羊的核苷酸序列与人、牛、猪、大鼠、鸡相比存在1处特有变异，氨基酸序列23～36存在高变异区。
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| 关 键 词： | 小尾寒羊 雌激素受体基因 克隆 序列分析 |
| 文章编号： | 1671-7236（2008）02-0060-04 |
| 收稿时间： | 2007-10-08 |
| 修稿时间： | 2007年10月8日 |
Cloning and Sequence Analysis of Exon 4 of Estrogen Receptor Gene in Small Tail Han Sheep |
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| JIA Li-hua,CHU Ming-xing,CHEN Hong-quan,FANG Li.Cloning and Sequence Analysis of Exon 4 of Estrogen Receptor Gene in Small Tail Han Sheep[J].China Animal Husbandry & Veterinary Medicine,2008,35(2):60-63. |
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| Authors: | JIA Li-hua CHU Ming-xing CHEN Hong-quan FANG Li |
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| Institution: | 1. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;2. The Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China |
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| Abstract: | According to the human,chicken and rat sequence of exon 4 of estrogen receptor(ESR) gene,a pair of primer was designed.DNA fragment of exon 4 of Small Tail Han sheep ESR gene was amplified successfully by PCR,and was cloned into pGEM-T Easy vector.The positive clones were further identified by PCR.The nucleotide sequence was detected and the amino acid sequence of this fragment was deduced.The comparison of the fragment of exon 4 of Small Tail Han sheep ESR gene with that of human,cattle,pig,rat and chicken ESR gene showed that the homologies of the nucleotide sequence and amino acid sequence of the fragment with human,cattle,pig,rat and chicken were from 77.68% to 97.28% and from 71.82% to 98.18%,respectively,which indicated that the ESR gene was highly conserved.Nucleotide sequence existed one special variation and the peptide sequence of amino acids 23 to 36 was highly variable in Small Tail Han sheep. |
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| Keywords: | Small Tail Han sheep estrogen receptor gene cloning sequence analysis |
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