Haematological and biochemical alterations in Korean catfish, Silurus asotus, experimentally infected with Edwardsiella tarda |
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| Authors: | Jin Ha Yu Jung Jo Han & Sung Woo Park |
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| Institution: | Department of Aquatic Life Medicine, Kunsan National University, Gunsan, Jeollabukdo, Korea;
Gyeonggi Province Freshwater Fisheries Researches Institute, Yangpyeong-gun, Gyeonggido, Korea |
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| Abstract: | This study determined the haematological changes in Korean catfish, Silurus asotus , experimentally infected with Edwardsiella tarda . Twenty-four or 48 h after infection with 1 × 104 colony-forming units (CFU) mL?1 of E. tarda , the fish were anaesthetized and the blood was collected. The haematological and biochemical tests included haematocrit (Ht), haemoglobin (Hb), leucocyte counts (Lc), total calcium (Ca), total protein (TP) concentration, alkaline phosphatase (ALP), glucose (GLU), aspartate aminotransferase, lysozyme and differential leucocyte populations. After 24- and 48-h infection, the values of Ht, Hb, Lc, TP, ALP and GLU were significantly decreased with respect to the control. Aspartate aminotransferase and lysozyme activity in both the 24- and 48-h groups exhibited higher levels compared with those in the control. However, the bacterial infection did not induce any significant alteration in the Ca concentration. After the 48-h infection, neutrophils and macrophage populations were reduced whereas lymphocytes were increased with respect to those in the control and the 24-h infection groups. The present study demonstrates that E. tarda infection causes haemato-biochemical responses that are related to the general physiological condition and defence system in catfish. |
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| Keywords: | Edwardsiella tarda haematology biochemistry catfish Silurus asotus |
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