| 巴西橡胶树乙醇酸氧化酶HbGOX1基因的鉴定与表达分析 |
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| 引用本文： | 程 汉,陈 相,黄华孙.巴西橡胶树乙醇酸氧化酶HbGOX1基因的鉴定与表达分析[J].热带作物学报,2016,36(8):19-25. |
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| 作者姓名： | 程 汉 陈 相 黄华孙 |
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| 作者单位： | 中国热带农业科学院橡胶研究所/国家橡胶树育种中心 海南儋州 571737;中国热带农业科学院橡胶研究所/国家橡胶树育种中心 海南儋州 571737;中国热带农业科学院橡胶研究所/国家橡胶树育种中心 海南儋州 571737 |
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| 摘 要： | 光呼吸途径是植物中重要的反应途径，它通过消耗光合作用产物影响作物的产量。乙醇酸氧化酶是该途径的关键调控酶，在橡胶树中尚未见研究报道。本研究分离和鉴定橡胶树HbGOX1基因的全长cDNA序列，对其编码的蛋白质进行生物信息学分析，并通过qPCR技术进一步研究HbGOX1基因在低温胁迫下的表达情况。结果发现，该基因受低温胁迫负调控。此结果为进一步揭示橡胶树光呼吸途径在橡胶合成中的作用奠定了基础。
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| 关 键 词： | 橡胶树 低温应答 光呼吸途径 HbGOX1基因 |
Identification and Expression of Glycolate Oxidase HbGOX1 Gene from Hevea brasiliensis |
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| Institution: | Rubber Research Institute, CATAS / State Center for Rubber Tree Breeding, Danzhou, Hainan 571737;Rubber Research Institute, CATAS / State Center for Rubber Tree Breeding, Danzhou, Hainan 571737;Rubber Research Institute, CATAS / State Center for Rubber Tree Breeding, Danzhou, Hainan 571737 |
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| Abstract: | Photorespiratory pathway is an important metabolic pathway in plant,which consumes the products of photo-synthesis and reduces crop final yield. Glycolate oxidase is the key regulatory enzyme of this pathway, while the study in rubber tree is absence. In this study, the cDNA sequence of HbGOX1 gene was identified and characterized, and the peptide sequence was analyzed bioinformatically. The expression pattern of HbGOX1 gene was further explored under cold stress. The results showed that this gene was negatively regulated by cold stress. This study provided some fundenmental information for the roles of photorespiration in rubber biosynthesis in Hevea brasiliensis. |
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| Keywords: | rubber tree cold responding photo-respiration pathway HbGOX1 gene |
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