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| Production of Transgenic Tall Fescue Plants with Enhanced Stress Tolerances by Agrobacterium tumefaciens-Mediated Transformation | |
| 作者姓名： | WU Guan-ting CHEN Jin-qing HU Zhang-hua LANG Chun-xiu CHEN Xiao-yun WANG Fu-lin JIN Wei XIA Ying-wu |
| 作者单位： | [1]Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R. China [2]Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, P.R. China |
| 基金项目： | 浙江省"151"人才工程基金 |
| 摘 要： | In order to improve stress tolerances of turf-type tall fescue （Festuca arundinacea Schreb.）, Agrobacterium tumefaciens strain EHA105 carrying plasmid pCMD containing stress tolerance-related CBF1 gene from Arabidopsis thaliana was used to transform mature seeds-derived embryogenic calli of four cultivars. A total of 112 transgenic plants were regenerated from 32 independent lines and verified by histochemical detection of GUS activity, PCR assay and Southern hybridization analysis. The transformation frequency ranged from 0.92 to 2.87% with apparent differences among the cultivars. Stress tolerances of transgenic plants were enhanced, which was shown by the facts that transgenic plants had distinct growth superiority and significantly higher survival rate than non-transformed ones under high salinity and high osmosis stresses, and that relative electronic conductivity of in vitro leaves treated with low and high temoeratures, dehvdration and high salinity stresses was 25-30% lower in transgenic plants than in control plants.In addition,it was observed that growth of transgenic plants was inhibited due to constitutive overexpression of CBF1 gene under normal environmental conditions. |
| 关 键 词： | 牛毛草 基因变化 温度 生长速度 |
Production of Transgenic Tall Fescue Plants with Enhanced Stress Tolerances by Agrobacterium tumefaciens-Mediated Transformation |
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| WU Guan-ting CHEN Jin-qing HU Zhang-hua LANG Chun-xiu CHEN Xiao-yun WANG Fu-lin JIN Wei XIA Ying-wu.Production of Transgenic Tall Fescue Plants with Enhanced Stress Tolerances by Agrobacterium tumefaciens-Mediated Transformation[J].Agricultural Sciences in China,2006,5(5):330-338. | |
| Authors: | WU Guan-ting CHEN Jin-qing HU Zhang-hua LANG Chun-xiu CHEN Xiao-yun WANG Fu-lin JIN Wei XIA Ying-wu |
| Institution: | 1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R. China;Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, P.R. China 2. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R. China 3. Institute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310029, P.R. China |
| Abstract: | In order to improve stress tolerances of turf-type tall fescue (Festuca arundinacea Schreb.), Agrobacterium tumefaciens strain EHA105 carrying plasmid pCMD containing stress tolerance-related CBF1 gene from Arabidopsis thaliana was used to transform mature seeds-derived embryogenic calli of four cultivars. A total of 112 transgenic plants were regenerated from 32 independent lines and verified by histochemical detection of GUS activity, PCR assay and Southern hybridization analysis. The transformation frequency ranged from 0.92 to 2.87% with apparent differences among the cultivars. Stress tolerances of transgenic plants were enhanced, which was shown by the facts that transgenic plants had distinct growth superiority and significantly higher survival rate than non-transformed ones under high salinity and high osmosis stresses, and that relative electronic conductivity of in vitro leaves treated with low and high temperatures, dehydration and high salinity stresses was 25–30% lower in transgenic plants than in control plants. In addition, it was observed that growth of transgenic plants was inhibited due to constitutive overexpression of CBF1 gene under normal environmental conditions. |
| Keywords: | tall fescue (Festuca arundinacea Schreb ) Agrobacterium tumefaciens genetic transformation CBF1 gene stress tolerance |
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