| O型口蹄疫病毒结构蛋白VP1的原核表达与抗原性分析 |
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| 引用本文： | 徐程,陈亚波,方维焕.O型口蹄疫病毒结构蛋白VP1的原核表达与抗原性分析[J].动物医学进展,2007,28(6):14-18. |
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| 作者姓名： | 徐程 陈亚波 方维焕 |
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| 作者单位： | 浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,浙江杭州,310029 |
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| 摘 要： | 研究分析了O型口蹄疫病毒(FMDV)结构蛋白VP1与当前猪FMDV疫苗血清的免疫反应性.将VP1基因克隆至原核表达载体pET32c,并在大肠埃希菌BL21中得到了表达,Western blot分析表明该重组蛋白与豚鼠O型FMDV标准阳性血清具有良好免疫反应性.目的蛋白经纯化后用ELISA分析其与猪疫苗血清的免疫反应性,结果显示该重组VP1蛋白(rVP1)只能与部分O型FMDV疫苗血清反应.推测当前使用的不同O型FMDV疫苗毒株在VP1重要中和抗原位点G-H环(134 aa～158 aa)与C末端(200 aa～213 aa)存在较大差异.
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| 关 键 词： | O型口蹄疫病毒 结构蛋白VP1 免疫反应性 疫苗毒株 中和抗原位点 |
| 文章编号： | 1007-5038（2007）06-0014-05 |
| 修稿时间： | 2007-01-16 |
Prokaryotic Expression of Structural Protein VP1 of Foot-and-mouth disease virus Type O and Analysis of Its Antigenicity |
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| XU Cheng,CHEN Ya-bo,FANG Wei-huan.Prokaryotic Expression of Structural Protein VP1 of Foot-and-mouth disease virus Type O and Analysis of Its Antigenicity[J].Progress In Veterinary Medicine,2007,28(6):14-18. |
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| Authors: | XU Cheng CHEN Ya-bo FANG Wei-huan |
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| Institution: | Institute of Preventive Veterinary Medicine of Zhejiang University and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, Zhejiang, 310029, China |
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| Abstract: | In this study,the immunoreactivity of structural protein VP1 of foot-and-mouth disease virus(FMDV) type O with sera from swine vaccinated against FMDV was analyzed.The gene encoding VP1 was cloned into the prokaryotic expression vector pET32c and expressed in E.coli BI21.Western blot analysis showed the recombinant protein had good immunoactivity with the reference serum from guinea pig against type O FMDV.ELISA analysis indicated that the purified VP1 protein was only reactive to part of the serum samples from swines immunized with inactivated FMDV type O vaccine,suggesting marked difference of several critical amino acids in the main neutralizing antigenic site of VP1 composed of G-H loop(residues 134-158) and C-terminus(residues 200-213) among current vaccine strains. |
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| Keywords: | Foot-and-mouth disease virus type O structural protein VP1 immunoreactivity vaccine strains neutralizing antigenic site |
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