| 赤楠叶黄酮类化合物的提取及其体外抗菌活性研究 |
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| 引用本文： | 黄晓昆,黄晓冬.赤楠叶黄酮类化合物的提取及其体外抗菌活性研究[J].安徽农业科学,2008,36(13):5257-5258. |
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| 作者姓名： | 黄晓昆 黄晓冬 |
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| 作者单位： | 泉州师范学院教育科学学院,福建泉州,362000 |
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| 摘 要： | 目的]探讨赤楠叶黄酮类化合物用作植物源农药的可行性。方法]采用微波处理、碱溶酸析等方法对赤楠叶黄酮进行提纯,用管碟法测定赤楠叶黄酮类化合物对7种细菌的抗菌活性,并测定其相应最小抑菌浓度(MIC)。结果]粗黄酮粉末得率为15.72%,其纯度约为90.65%。100 g/L赤楠叶黄酮类化合物对藤黄八叠球菌、金黄色葡萄球菌、白菜软腐菌与普通变形杆菌的抑菌圈直径均大于15mm,特别是对藤黄八叠球菌、金黄色葡萄球菌高达31.3与22.5 mm。藤黄八叠球菌的MIC最小(25 g/L);金黄色葡萄球菌与白菜软腐菌次之(50 g/L);其余各菌均为100 g/L。结论]用微波处理、碱溶酸析、聚酰胺吸附洗脱等方法提纯赤楠叶黄酮简便可行。赤楠叶黄酮类化合物对不同菌种的抑菌活性不同。
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| 关 键 词： | 赤楠 叶 黄酮类 提取 抗菌活性 |
Study on the Extraction and Antibacterial Activity in Vitro of Flavonoids from Syzygium buxifolium Leaves |
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| HUANG Xiao-kun et al.Study on the Extraction and Antibacterial Activity in Vitro of Flavonoids from Syzygium buxifolium Leaves[J].Journal of Anhui Agricultural Sciences,2008,36(13):5257-5258. |
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| Authors: | HUANG Xiao-kun |
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| Abstract: | Objective] The aim of the study was to discuss the feasibility of using flavoniods from Syzygium buxifolium leaves as botanical pesticide.Method] The flavoniods were extracted and purified by the method of microwave treatment,alkali-extraction and acid-precipitation from S.buxifolium leaves.Its antibacterial activities on 7 bacterial species were determined by cylinder-plate method and their corresponding minimum inhibition concn.(MIC) were measured.Result] The yield rate of crude flavonoids powder was 15.72%,with purity of 90.65%.The antibacterial circle diameters of flavoniods from S.buxifolium leaves at 100 g/L on Sarcina lutea,Staphylococcus aureus,Erwinia carotorora and Proteus vulgaris were bigger than 15 mm,especially on S.lutea and S.aureus,being up to 31.3 and 22.5 mm.The MIC of S.lutea was smallest(25 g/L),that of S.aureus and E.carotorora were secondary(50 g/L) and that of the other bacteria were 100 g/L.Conclusion] Extraction and purification of flavonoids from S.buxifolium leaves by the method of microwave treatment,alkali-extraction and acid-precipitation, adsorption and elution by polyamide was simple,convenient and feasible.The flavonoids from S.buxifolium leaves had different antibacterial activities on different bacterial species. |
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| Keywords: | Syzygium buxifolium Leaves Flavonoids Extraction Antibacterial activity |
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