| 响应面法优化枯草芽孢杆菌BS-1101原生质体制备条件 |
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| 引用本文： | 姚建波,吴志军,陈志宝.响应面法优化枯草芽孢杆菌BS-1101原生质体制备条件[J].黑龙江八一农垦大学学报,2011,23(6):44-48. |
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| 作者姓名： | 姚建波 吴志军 陈志宝 |
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| 作者单位： | 黑龙江八一农垦大学生命科学技术学院,大庆,163319 |
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| 摘 要： | 实验以枯草芽孢杆菌BS-1101原生质体的制备率为目的,通过中心组合试验对影响枯草芽孢杆菌BS-1101原生质体的制备率的三个因素：酶解温度、酶解时间、酶浓度进行了研究评价,通过中心组合试验设计与响应面分析方法得到二次回归模型程,由回归方程得到,在酶解温度37.27℃、酶浓度为5.152 mg.mL-1、酶解时间88....
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| 关 键 词： | 枯草芽孢菌 原生质体 响应面法 |
Optimization of Protoplast Formation Conditions of Bacillus Subtilis BS-1101 by Using Response Surface Methodology |
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| Yao Jianbo,Wu Zhijun,Chen Zhibao.Optimization of Protoplast Formation Conditions of Bacillus Subtilis BS-1101 by Using Response Surface Methodology[J].Journal of Heilongjiang August First Land Reclamation University,2011,23(6):44-48. |
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| Authors: | Yao Jianbo Wu Zhijun Chen Zhibao |
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| Institution: | (College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319) |
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| Abstract: | The central composite design combined with response surface methodology was employed to optimize the protoplast formation conditions of Bacillus subtilis BS-1101.The critical parameters,such as lysozyme concentration,temperature,and contact time which affects the formation of protoplast of the Bacillus subtilis BS-1101 were studied using a novel technique named response surface methodology.Second-order multiple regression analysis was used to build a predicted model of the combined effects of independent variables(enzyme concentration,temperature and effect time) for maximum the yield of protoplast of Bacillus subtilis BS-1101.The optimal parameters to obtain the highest protoplast yield were as followed: temperature was 37.27 ℃,effect time was 88.8min,enzyme concentration was 5.152 mg·mL-1.The average experimental protoplast yield under the optimum conditions was found to be 77.02%,which agreed with the predicted value.The proposed method showed high degree of reproducibility.The central composite design was employed to optimize the protoplast formation conditions of Bacillus subtilis BS-1101. |
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| Keywords: | bacillus subtilis protoplast response surface methodology |
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