| 甜菜细胞质雄性不育与内源激素含量的关系 |
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| 引用本文： | 王华忠,吴则东,韩英,方智远.甜菜细胞质雄性不育与内源激素含量的关系[J].中国农业科学,2008,41(4):1134-1141. |
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| 作者姓名： | 王华忠 吴则东 韩英 方智远 |
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| 作者单位： | 1. 黑龙江省普通高校甜菜遗传育种重点实验室/黑龙江大学,哈尔滨,150080;中国农业科学院甜菜研究所/黑龙江大学农作物研究院,哈尔滨,150080
2. 中国农业科学院蔬菜花卉研究所,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 【目的】研究甜菜细胞质雄性不育与内源激素含量的关系。【方法】以表现型具明显差异的两对甜菜单胚CMS系及其O型系作为试验材料，采用间接酶联免疫（ELISA）检测技术，分析营养生长期和生殖生长期的功能叶片和花蕾中生长素、细胞分裂素、脱落酸、赤霉素等4大类内源激素的含量和比值变化。【结果】在营养生长的糖分积累期，ABA、IAA、GA3含量均为O型系高于CMS系，差异显著，而DHZR、IPA、ZR含量在营养生长期间均差异不显著；在开花初期的叶片，除IPA外，IAA、ABA、DHZR、ZR、GA3含量均为O型系高于CMS系；饱满花蕾的IAA、ABA、ZR、GA3含量均为O型系高于CMS系。甜菜激素变化较有规律的时期和器官依次为开花盛期的饱满花蕾、开花初期的叶片和和营养生长阶段糖分积累期的叶片。在上述3个时期， CMS系与O型系甜菜的激素含量变化较大，CMS系的IAA/ABA、ZR/ABA和GA3/ABA值分别为O型系的0.55倍、1.43倍、1.64倍；2.66倍、1.55倍、1.49倍和1.14倍、1.60倍、1.53倍。【结论】各种激素的含量变化与甜菜CMS相关密切。影响甜菜CMS的时期、器官和激素比值分别为饱满花蕾IAA/ABA的降低、ZR/ABA和GA3/ABA的提高，开花初期和糖分积累期的叶片为IAA/ABA、ZR/ABA和GA3/ABA的提高。该研究为探讨甜菜细胞质雄性不育的生理机制和育性的调控提供了理论依据。
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| 关 键 词： | 甜菜 细胞质雄性不育 生育周期 内源激素 |
| 收稿时间： | 2006-7-20 |
| 修稿时间： | 2006年8月9日 |
Relationships Between Endogenous Hormone Contents and Cytoplasmic Male Sterility in Sugarbeet |
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| WANG Hua-zhong,WU Ze-dong,HAN Ying,FANG Zhi-yuan.Relationships Between Endogenous Hormone Contents and Cytoplasmic Male Sterility in Sugarbeet[J].Scientia Agricultura Sinica,2008,41(4):1134-1141. |
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| Authors: | WANG Hua-zhong WU Ze-dong HAN Ying FANG Zhi-yuan |
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| Abstract: | Two pair of monogerm cytoplasmic male sterile (CMS) and maintenance line sugar beet with significantly different phynotype were chosen in this study. Enzyme-linked immunosorbent assays (ELISA) was used for testing change regularity of endogenous hormone, indoleacetic acid (IAA), cytokinin, abscisic acid (ABA) and gibberellin (GA3) in leaf and bud of vegetative growth and reproductive growth period. The results showed that in vegetative growth period, the IAA and isopentenylribosidoadenine(IPA) content of maintenance line leaf were higher than that of CMS sugar beet, and but contrary results of ABA and ribosidoadenine(ZR) content in leaf fascicle formation stage. Definite regularity of all hormones was not found in maintenance and CMS sugar beet leaf line in root epacme stage. The IAA and GA3 content of maintenance line leaf were higher than that of CMS sugar beet in sugar accumulation stage. In reproductive growth period, the GA3 content of maintenance line leaf was higher than that of CMS sugar beet in leaf rosulate fascicle stage. The IAA and GA3 content of CMS line leaf were higher than that of maintenance line sugar beet in bolting stage. The IAA, ABA, dihydroribosidoadenine (DHZR) and GA3 content of maintenance line leaf were higher than that of CMS sugar beet in early flowering stage. The ABA, DHZR, IPA and GA3 of maintenance line were higher than that of CMS sugar beet except ZR in bud. The study of change relation CMA sugar beet with endogenous hormone will help to know physiological mechanism and utilization of cytoplasmic male sterile sugar beet. |
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| Keywords: | Sugar beet Cytoplasmic male sterility Developmental cycle Endogenous hormone Change regularity |
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