Enzyme-linked immunosorbent assay (ELISA) for the detection of potato viruses A and Y in potato leaves and sprouts |
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| Authors: | D Z Maat J A De Bokx |
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| Institution: | 1. Instituut voor Plantenziektenkundig Onderzoek, Postbus 42, 6700 AA, Wageningen, the Netherlands
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| Abstract: | With the enzyme-linked immunosorbent assay (ELISA) potato virus A (PVA) could be detected reliably in potato sprouts, especially when these were young and sappy. The detection of this virus in leaves of glasshouse-grown potato plants was less reliable. The tobacco veinal necrosis strain of potato virus Y (PVYN) was readily demonstrated in foliage of glass-house-grown potato plants using an antiserum to this strain. Plants infected with the common strain (PVYO) did not react in ELISA with this antiserum. In young sappy sprouts, using the PVYN antiserum, PVYN could be detected reliably when samples with PVYO were excluded, as the reaction of samples infected with the latter virus was intermediate between PVYN-diseased and PVY-free samples. PVY was also detected in plants inadvertently infected during the experiments. |
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