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| 4个两系杂交稻亲本的SSR多态性分析 | |
| 引用本文： | 戴剑,洪德林,吴燕,陈兰.4个两系杂交稻亲本的SSR多态性分析[J].南京农业大学学报,2011,34(3). |
| 作者姓名： | 戴剑 洪德林 吴燕 陈兰 |
| 作者单位： | 1. 南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095;江苏省农业科学院粮食作物研究所,江苏,南京,210014 2. 南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095 3. 江苏省农业科学院粮食作物研究所,江苏,南京,210014 |
| 基金项目： | 国家863计划项目(2010AA101301); 江苏省农业科学院科研基金项目(6110830) |
| 摘 要： | 用52对SSR引物对以培矮64S为母本的4个两系杂交稻组合的5个亲本DNA多态性进行了分析,并以1个杂交粳稻86优8号作为对照。结果表明:46对引物能在7个亲本间扩增出多态性条带;8对引物能将4个两系杂交稻与对照杂交粳稻区分开,且在86优8号的亲本中具有多态性;可区分两优培九、两优108、培矮64S/E32、两优122和86优8号F1及其亲本的SSR引物分别为34、32、31、30和14对;有16对SSR引物可用于区分4个两系杂交组合F1和亲本,引物RM206和RM286可区分5个组合和各自的亲本。应用其中1对引物RM505对两优培九进行鉴定验证,结果表明该引物能准确区分两优培九及其亲本。根据本试验中引物的多态性和特异性结果,有多种途径可鉴别这4个两系杂交组合。可通过RM13(或RM206、RM286等)、RM224(或RM337)、RM234(或RM252、RM505和RM565)和RM25(或RM217、RM248和RM585)等4对引物将两优培九、两优108、培矮64S/E32和两优122分别加以鉴别。 |
| 关 键 词： | 两系杂交稻 SSR标记 多态性 |
Analysis of SSR polymorphism among parents of four two-linehybrids in rice(Oryza sativa L.) |
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| DAI Jian,HONG De-lin,WU Yan,CHEN Lan.Analysis of SSR polymorphism among parents of four two-linehybrids in rice(Oryza sativa L.)[J].Journal of Nanjing Agricultural University,2011,34(3). | |
| Authors: | DAI Jian HONG De-lin WU Yan CHEN Lan |
| Institution: | DAI Jian1,2,HONG De-lin1,WU Yan2,CHEN Lan1(1.State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China,2.Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China) |
| Abstract: | Sterility of female parent in two-line hybrid rice is often influenced by the illumination time and temperature.It results in selfing in sterile lines and reduces the quality of hybrid seed.In this study,DNA polymorphism of five parents of four two-line hybrid rice with Peiai64S as female parent were analyzed by 52 pairs of SSR primers with one japonica hybrid rice named 86you8 as the control.46 SSR primers could amplify polymorphism among 7 parents.8 pairs of SSR primers which could amplify polymorphism am... |
| Keywords: | two-line hybrid rice SSR marker polymorphism |
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