| 蝴蝶兰组织培养与快速繁殖技术研究 |
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| 引用本文： | 张雅晨,姜亚博,周烽明,顾季春,顾福根.蝴蝶兰组织培养与快速繁殖技术研究[J].现代农业科技,2011(23):254-256. |
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| 作者姓名： | 张雅晨 姜亚博 周烽明 顾季春 顾福根 |
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| 作者单位： | 苏州大学医学部,江苏苏州,215123 |
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| 基金项目： | 苏州大学第十三批大学生课外学术科研基金 |
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| 摘 要： | 以残败花梗为外植体进行组织培养,建立了蝴蝶兰的无菌繁殖体系,研究了培养基中不同种类植物生长调节物质及其浓度比例对休眠芽的萌发、丛生芽的诱导以及试管苗生根的影响,筛选出了最佳培养基组成;研究了活性炭、PVP、温度、暗培养对培养基褐化的影响。结果表明:在1/2 MS+6-BA 3.0 mg/L+NAA 0.2 mg/L+10%香蕉汁+3%蔗糖+0.6%琼脂的培养基上休眠芽的诱导效果最好,诱导率可达87.5%;在1/2 MS+6-BA 6.0 mg/L+NAA 0.1 mg/L+10%香蕉汁+3%蔗糖+0.6%琼脂的培养基上丛生芽的诱导效果最好,增殖系数可达3.15;在1/2 MS+NAA 0.5 mg/L+活性炭1 000 mg/L+3%蔗糖+0.6%琼脂的培养基上生根效果最好,生根率可达95%,平均每株生根3.11条左右,炼苗后移植成活率可达90%。
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| 关 键 词： | 蝴蝶兰 组织培养 快速繁殖 诱导 增殖 生根 |
Study on Tissue Culture and Rapid Propagation Technology of Phalaenopsis amabilis（L.）C.L.Blume |
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| Authors: | ZHANG Ya-chen JIANG Ya-bo ZHOU Feng-ming GU Ji-chun GU Fu-gen |
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| Institution: | (School of Medicine,Suzhou University,Suzhou Jiangsu 215123) |
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| Abstract: | Flower peduncles of Phalaenopsis amabilis（L.）C.L.Blume were cultured to induce seedlings on medium,then shoots were cultured as explants to produce shoot cluters in vitro.The effects of different plant growth regulators and their concentrations on the germination of dormant buds,induction of clusters buds and vitro rooting were studied to select the best medium.The effects of activated charcoal,PVP,temperature,dark culture on the medium browning were also investigated.The results showed that：the best media inducing dormant buds was 1/2 MS＋6-BA 3.0 mg/L＋NAA 0.2 mg/L＋10% banana juice＋3% sucrose＋0.6% agar,the induction rate of shoots was up to 87.5%.The best media inducing clusters buds was 1/2 MS＋6-BA 6.0 mg/L＋NAA 0.1 mg/L＋10% banana juice＋3% sucrose＋0.6% agar,the multiplication coefficient was up to 3.15.The best media inducing rooting was 1/2 MS＋NAA 0.5 mg/L＋1 000 mg/L activaed charcoal＋3% sucrose＋0.6% agar,the rooting rate reached to 95%,average number of roots was about 3.11 stripes/plant,transplant survival rate was 90% after hardening seedling. |
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| Keywords: | Phalaenopsis amabilis（L ）C L Blume tissue culture rapid propagation introduce multiplication rooting |
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