| 水稻稻瘟病拮抗稀有放线菌的筛选及防治效果 |
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| 引用本文： | 阮宏椿,石妞妞,杜宜新,陈福如.水稻稻瘟病拮抗稀有放线菌的筛选及防治效果[J].中国生物防治学报,2021,37(3):538-546. |
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| 作者姓名： | 阮宏椿 石妞妞 杜宜新 陈福如 |
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| 作者单位： | 福建省作物有害生物监测与治理重点实验室/福建省农业科学院植物保护研究所, 福州 350013 |
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| 基金项目： | 福建省属公益类科研院所专项（2019R1024-6）；福建省农业科学院创新平台专项（CXPT0022019005）；福建省农业科学院对外合作项目（DEC201908）；国家重点研发计划（2016YFD0300707） |
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| 摘 要： | 为获得对水稻稻瘟病菌具有良好抑菌效果的稀有放线菌，从红豆杉根际土壤样品中分离获得11株放线菌，筛选出对水稻稻瘟病菌抑菌效果最好的菌株ST7—2，抑菌率为79.88%，经形态特征、生理生化特征观察和16S rRNA、recA、gyrB、rpoB序列分析，确定菌株ST7—2为杀结节链霉菌Streptomyces tubercidicus。菌株ST7—2对水稻稻瘟病菌Magnaporthe oryzae、大豆炭疽病菌Colletotrichum truncatum、芦笋茎枯病菌Phomopsis asparagi、水稻稻曲病菌Ustilaginoidea virens、蘑菇褐腐病菌Mycogone perniciosa共5种病原真菌具有较好的抑菌效果。室内盆栽试验结果表明，菌株ST7—2发酵液对水稻苗瘟防效为82.76%，与75%三环唑可湿性粉剂1700倍无显著性差异；田间小区试验结果显示，发酵液对水稻穗颈瘟防效为73.57%，比75%三环唑WP 1700倍的防效略低。因此，杀结节链霉菌ST7—2是防治水稻稻瘟病的潜在生防菌株，生防应用前景广阔。
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| 关 键 词： | 稀有放线菌 水稻 稻瘟病菌 拮抗作用 分类鉴定 |
| 收稿时间： | 2020-11-03 |
Screening and Control Effect of Rare Antagonistic Actinomycetes against Rice Blast |
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| RUAN Hongchun,SHI Niuniu,DU Yixin,CHEN Furu.Screening and Control Effect of Rare Antagonistic Actinomycetes against Rice Blast[J].Chinese Journal of Biological Control,2021,37(3):538-546. |
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| Authors: | RUAN Hongchun SHI Niuniu DU Yixin CHEN Furu |
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| Institution: | Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China |
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| Abstract: | To screen the rare actinomycetes with good antifungal effect against the Magnaporthe oryzae. Actinomycetes were isolated from rhizosphere soil of Taxus chinensis. The results showed that a total of 11 strains of actinomycetes were isolated. Strain ST7?2 showed the best inhibitory effect on M. oryzae, and the inhibitory rate was 79.88%. ST7?2 was identified as Streptomyces tubercidicus combining 16S rRNA, recA, gyrB, rpoB sequence analysis, and the morphological, physiological, and biochemical characteristics. In addition, ST7?2 displayed good effects on Magnaporthe oryzae, Colletotrichum truncatum, Phomopsis asparagi, Ustilaginoidea virens and Mycogone perniciosa. The control effect of fermentation broth against seeding blast was 82.76% under the greenhouse, which showed no significant difference with that of 75% tricyclazole wettable powder 1700 fold. In field trials, the control effect of fermentation broth was 73.57%, which was slightly less effective than 75% tricyclazole WP. Therefore, S. tubercidicus ST7?2 is a potential biocontrol strain against rice blast, and show a wild prospect for development and application in the future. |
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| Keywords: | rare actinomycetes rice Magnaporthe oryzae antifungal effect taxonomic identification |
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